Shear force processing of lipoaspirates for stem cell enrichment does not affect secretome of human cells detected by mass spectrometry in vitro

L Prantl, A Eigenberger, S Klein, K Limm… - Plastic and …, 2020 - journals.lww.com
Background: Lipofilling is one of the most often performed surgical procedures in plastic and
reconstructive surgery. Lipoaspirates provide a ready source of stem cells and secreted …

Low osmolality and shear stress during liposuction impair cell viability in autologous fat grafting

T Ismail, J Bürgin, A Todorov, R Osinga, N Menzi… - Journal of Plastic …, 2017 - Elsevier
Background Liposuction and subsequent autologous fat grafting have become essential
techniques for fat augmentation in plastic surgery. However, standard harvesting techniques …

Sources of processed lipoaspirate cells: influence of donor site on cell concentration

AV Padoin, J Braga-Silva, P Martins… - Plastic and …, 2008 - journals.lww.com
Background: Recently, adipose tissue harvested by liposuction has been identified as a
source of processed lipoaspirate cells. The aim of this study was to determine the …

Processing technique for lipofilling influences adipose-derived stem cell concentration and cell viability in lipoaspirate

M Pfaff, W Wu, E Zellner, DM Steinbacher - Aesthetic plastic surgery, 2014 - Springer
Background Autologous fat grafting is a highly used technique in plastic and reconstructive
surgery. Several fat-processing techniques have been described, with centrifugation …

In vitro validation of a closed device enabling the purification of the fluid portion of liposuction aspirates

C Cicione, G Di Taranto, M Barba… - Plastic and …, 2016 - journals.lww.com
Background: Adipose tissue harvested through lipoaspiration is widely exploited in plastic
and cosmetic surgery, because of its remarkable trophic properties, especially relying on the …

Adipose tissue-derived stem cell yield depends on isolation protocol and cell counting method

L Prantl, A Eigenberger, E Brix, S Kempa, M Baringer… - Cells, 2021 - mdpi.com
In plastic surgery, lipofilling is a frequent procedure. Unsatisfactory vascularization and
impaired cell vitality can lead to unpredictable take rates in the fat graft. The proliferation and …

Is centrifugation necessary for processing lipoaspirate harvested via water-jet force assisted technique before grafting? Evidence of lipoaspirate concentration with …

S Yin, J Luan, S Fu, Q Zhuang - Annals of Plastic Surgery, 2016 - journals.lww.com
Background Although water-jet force–assisted liposuction technique (WAL) was
demonstrated to have favorable effects on fat grafting, controversy continues concerning the …

Influences of preservation at various temperatures on liposuction aspirates

D Matsumoto, T Shigeura, K Sato, K Inoue… - Plastic and …, 2007 - journals.lww.com
Background: Aspirated fat is not only a filler material but also an abundant source of adipose-
derived stem cells. The aim of this study was to assess degeneration of aspirated fat during …

Enhancement of viable adipose-derived stem cells in lipoaspirate by buffering tumescent with sodium bicarbonate

A Francis, WZ Wang, JJ Goldman… - … Surgery–Global Open, 2019 - journals.lww.com
Background: Fat grafting is a growing field within plastic surgery. Adipose-derived stem cells
(ASCs) and stromal vascular fracture (SVF) may have a role in fat graft survival. Our group …

Enhancement of progenitor cells by two-step centrifugation of emulsified lipoaspirates

N Pallua, J Grasys, BS Kim - Plastic and reconstructive surgery, 2018 - journals.lww.com
Background: Adipose-derived stem cells, endothelial progenitor cells, and soluble factors
jointly contribute to the regenerative effect of fat grafts. Nanofat grafting emulsifies the …