Real-time PCR: revolutionizing detection and expression analysis of genes

SA Deepak, KR Kottapalli, R Rakwal, G Oros… - Current …, 2007 - ingentaconnect.com
Invention of polymerase chain reaction (PCR) technology by Kary Mullis in 1984 gave birth
to real-time PCR. Real-time PCR-detection and expression analysis of gene (s) in real-time …

Real-time PCR and its application for rapid plant disease diagnostics

NW Schaad, RD Frederick - Canadian journal of plant pathology, 2002 - Taylor & Francis
Rapid-cycle real-time polymerase chain reaction (PCR) methods may revolutionize the
manner in which plant pathogens are identified and diseases are diagnosed. As the …

Real-time PCR: what relevance to plant studies?

C Gachon, A Mingam, B Charrier - Journal of experimental …, 2004 - academic.oup.com
The appearance of genetically modified organisms on the food market a few years ago, and
the demand for more precise and reliable techniques to detect foreign (transgenic or …

[图书][B] Real-time PCR: an essential guide.

K Edwards, J Logan, N Saunders - 2004 - cabidigitallibrary.org
Real-time polymerase chain reaction (PCR) is a powerful and rapid technique for nucleic
acid amplification. This book presents a comprehensive guide to the most appropriate and …

Quantification using real-time PCR technology: applications and limitations

D Klein - Trends in molecular medicine, 2002 - cell.com
The introduction of real-time PCR technology has significantly improved and simplified the
quantification of nucleic acids, and this technology has become an invaluable tool for many …

[图书][B] Real-time PCR

MT Dorak - 2007 - books.google.com
With a variety of detection chemistries, an increasing number of platforms, multiple choices
for analytical methods and the jargon emerging along with these developments, real-time …

Real-time polymerase chain reaction: applications to studies on soilborne pathogens

PA Okubara, KL Schroeder… - Canadian Journal of Plant …, 2005 - Taylor & Francis
Real-time, or quantitative, polymerase chain reaction (Q-PCR), offers a rapid, sensitive, and
specific method for the diagnosis of plant pathogens in soil, water, air, and plant samples …

The real-time polymerase chain reaction

M Kubista, JM Andrade, M Bengtsson… - Molecular aspects of …, 2006 - Elsevier
The scientific, medical, and diagnostic communities have been presented the most powerful
tool for quantitative nucleic acids analysis: real-time PCR [Bustin, SA, 2004. A–Z of …

High-resolution semi-quantitative real-time PCR without the use of a standard curve

A Gentle, F Anastasopoulos, NA McBrien - Biotechniques, 2001 - Taylor & Francis
The repeatability and sensitivity of a simple, adaptable, semi-quantitative, realtime RT-PCR
assay was investigated. The assay can be easily and rapidly applied to quantitate relative …

Minimum information necessary for quantitative real-time PCR experiments

G Johnson, AA Nour, T Nolan, J Huggett… - Quantitative Real-Time …, 2014 - Springer
The MIQE (minimum information for the publication of quantitative real-time PCR) guidelines
were published in 2009 with the twin aims of providing a blueprint for good real-time …