Miniaturized continuous-flow digital PCR for clinical-level serum sample based on the 3D microfluidics and CMOS imaging device
B Li, Y Li, A Manz, W Wu - Sensors, 2020 - mdpi.com
In recent years, the development of polymerase chain reaction (PCR) technology has
focused on digital PCR, which depends on the microfluidics. Based on continuous-flow …
focused on digital PCR, which depends on the microfluidics. Based on continuous-flow …
Compressed air-driven continuous-flow thermocycled digital PCR for HBV diagnosis in clinical-level serum sample based on single hot plate
K Wang, B Li, W Wu - Molecules, 2020 - mdpi.com
We report a novel compressed air-driven continuous-flow digital PCR (dPCR) system based
on a 3D microfluidic chip and self-developed software system to realize real-time monitoring …
on a 3D microfluidic chip and self-developed software system to realize real-time monitoring …
A size reduction method for rapid digital PCR using thin-film chip and vacuum pouch microfluidic system
The digital polymerase chain reaction (dPCR) is a high efficacy detection method that is
widely applied in lab-on-a-chip systems. Reported dPCR microfluidic devices usually …
widely applied in lab-on-a-chip systems. Reported dPCR microfluidic devices usually …
Experimental investigation of confinement effect in single molecule amplification via real-time digital PCR on a multivolume droplet array SlipChip
Background Digital polymerase chain reaction (digital PCR) is an important quantitative
nucleic acid analysis method in both life science research and clinical diagnostics. One …
nucleic acid analysis method in both life science research and clinical diagnostics. One …
A droplet-based multivolume microfluidic device for digital polymerase chain reaction
Digital PCR plays an essential role in detecting and quantifying nucleic acid targets. At the
same time, there are still challenges in its applications, including reduction of cost …
same time, there are still challenges in its applications, including reduction of cost …
A rapid digital pcr system with a pressurized thermal cycler
We designed a silicon-based fast-generated static droplets array (SDA) chip and developed
a rapid digital polymerase chain reaction (dPCR) detection platform that is easy to load …
a rapid digital polymerase chain reaction (dPCR) detection platform that is easy to load …
A three-in-one microfluidic droplet digital PCR platform for absolute quantitative analysis of DNA
Droplet digital polymerase chain reaction (ddPCR) technology has found widespread
applications in the ultrasensitive analysis of nucleic acids, where integrated ddPCR …
applications in the ultrasensitive analysis of nucleic acids, where integrated ddPCR …
Lower fluidic resistance of double-layer droplet continuous flow PCR microfluidic chip for rapid detection of bacteria
Z Li, Y Wang, Z Gao, S Sekine, Q You, S Zhuang… - Analytica Chimica …, 2023 - Elsevier
Background Rapid diagnosis of harmful microorganisms demonstrated its great importance
for social health. Continuous flow PCR (CF-PCR) can realize rapid amplification of target …
for social health. Continuous flow PCR (CF-PCR) can realize rapid amplification of target …
Convenient microfluidic cartridge for single-molecule droplet PCR using common laboratory equipment
H Takahara, H Matsushita, E Inui, M Ochiai… - Analytical …, 2021 - pubs.rsc.org
We have previously established a cost-efficient in-house system for single-molecule droplet
polymerase chain reaction (PCR) using a polydimethylsiloxane microfluidic cartridge and …
polymerase chain reaction (PCR) using a polydimethylsiloxane microfluidic cartridge and …
An integrated droplet digital PCR gene chip for absolute quantification of nucleic acid
X Meng, Y Yu, P Gong, G Jin - Microfluidics and Nanofluidics, 2021 - Springer
An integrated droplet digital polymerase chain reaction (ddPCR) gene chip that can quantify
nucleic acid absolutely was constructed to avoid problems such as droplet fusion …
nucleic acid absolutely was constructed to avoid problems such as droplet fusion …