Miniaturized continuous-flow digital PCR for clinical-level serum sample based on the 3D microfluidics and CMOS imaging device

B Li, Y Li, A Manz, W Wu - Sensors, 2020 - mdpi.com
In recent years, the development of polymerase chain reaction (PCR) technology has
focused on digital PCR, which depends on the microfluidics. Based on continuous-flow …

Compressed air-driven continuous-flow thermocycled digital PCR for HBV diagnosis in clinical-level serum sample based on single hot plate

K Wang, B Li, W Wu - Molecules, 2020 - mdpi.com
We report a novel compressed air-driven continuous-flow digital PCR (dPCR) system based
on a 3D microfluidic chip and self-developed software system to realize real-time monitoring …

A size reduction method for rapid digital PCR using thin-film chip and vacuum pouch microfluidic system

CJ Lee, YH Hsu - Microfluidics and Nanofluidics, 2022 - Springer
The digital polymerase chain reaction (dPCR) is a high efficacy detection method that is
widely applied in lab-on-a-chip systems. Reported dPCR microfluidic devices usually …

Experimental investigation of confinement effect in single molecule amplification via real-time digital PCR on a multivolume droplet array SlipChip

Y Luo, Q Hu, Y Yu, W Lyu, F Shen - Analytica Chimica Acta, 2024 - Elsevier
Background Digital polymerase chain reaction (digital PCR) is an important quantitative
nucleic acid analysis method in both life science research and clinical diagnostics. One …

A droplet-based multivolume microfluidic device for digital polymerase chain reaction

C Wei, C Yu, S Li, J Meng, T Li, J Cheng, J Li - Sensors and Actuators B …, 2022 - Elsevier
Digital PCR plays an essential role in detecting and quantifying nucleic acid targets. At the
same time, there are still challenges in its applications, including reduction of cost …

A rapid digital pcr system with a pressurized thermal cycler

X Chen, Q Song, B Zhang, Y Gao, K Lou, Y Liu, W Wen - Micromachines, 2021 - mdpi.com
We designed a silicon-based fast-generated static droplets array (SDA) chip and developed
a rapid digital polymerase chain reaction (dPCR) detection platform that is easy to load …

A three-in-one microfluidic droplet digital PCR platform for absolute quantitative analysis of DNA

Y Ren, J Ji, H Zhang, L Cao, J Hu, F Xu, Z Li - Lab on a Chip, 2023 - pubs.rsc.org
Droplet digital polymerase chain reaction (ddPCR) technology has found widespread
applications in the ultrasensitive analysis of nucleic acids, where integrated ddPCR …

Lower fluidic resistance of double-layer droplet continuous flow PCR microfluidic chip for rapid detection of bacteria

Z Li, Y Wang, Z Gao, S Sekine, Q You, S Zhuang… - Analytica Chimica …, 2023 - Elsevier
Background Rapid diagnosis of harmful microorganisms demonstrated its great importance
for social health. Continuous flow PCR (CF-PCR) can realize rapid amplification of target …

Convenient microfluidic cartridge for single-molecule droplet PCR using common laboratory equipment

H Takahara, H Matsushita, E Inui, M Ochiai… - Analytical …, 2021 - pubs.rsc.org
We have previously established a cost-efficient in-house system for single-molecule droplet
polymerase chain reaction (PCR) using a polydimethylsiloxane microfluidic cartridge and …

An integrated droplet digital PCR gene chip for absolute quantification of nucleic acid

X Meng, Y Yu, P Gong, G Jin - Microfluidics and Nanofluidics, 2021 - Springer
An integrated droplet digital polymerase chain reaction (ddPCR) gene chip that can quantify
nucleic acid absolutely was constructed to avoid problems such as droplet fusion …