Synthetic sugar cassettes for the efficient production of flavonol glycosides in Escherichia coli

P Parajuli, RP Pandey, NTH Trang, AK Chaudhary… - Microbial cell …, 2015 - Springer
Background A multi-monocistronic synthetic vector was used to assemble multiple genes of
a nucleotide diphosphate (NDP)-sugar biosynthetic pathway to construct robust genetic …

Biosynthesis of flavone C-glucosides in engineered Escherichia coli

A Shrestha, RP Pandey, D Dhakal, P Parajuli… - Applied microbiology …, 2018 - Springer
Two plant-originated C-glucosyltransferases (CGTs) UGT708D1 from Glycine max and
GtUF6CGT1 from Gentiana triflora were accessed for glucosylation of selected flavones …

Metabolic engineering of Escherichia coli for the biological synthesis of 7-O-xylosyl naringenin

D Simkhada, EM Kim, HC Lee, JK Sohng - Molecules and cells, 2009 - Springer
Flavonoids are a group of polyphenolic compounds that have been recognized as important
due to their physiological and pharmacological roles and their health benefits. Glycosylation …

Biosynthesis and production of glycosylated flavonoids in Escherichia coli: current state and perspectives

BG Kim, SM Yang, SY Kim, MN Cha, JH Ahn - Applied Microbiology and …, 2015 - Springer
Flavonoids are plant secondary metabolites containing several hydroxyl groups that are
targets for modification reactions such as methylation and glycosylation. In plants, flavonoids …

Exploration of glycosylated flavonoids from metabolically engineered E. coli

D Simkhada, NP Kurumbang, HC Lee… - Biotechnology and …, 2010 - Springer
Flavonoids glycosylated with UDP-glucuronic acid and UDP-xylose are spatially distributed
in nature. To produce these glycosides, E. coli was engineered to overexpress biosynthetic …

Metabolic engineering of Escherichia coli for the biosynthesis of flavonoid-O-glucuronides and flavonoid-O-galactoside

SY Kim, HR Lee, K Park, BG Kim, JH Ahn - Applied microbiology and …, 2015 - Springer
Most flavonoids are glycosylated and the nature of the attached sugar can strongly affect
their physiological properties. Although many flavonoid glycosides have been synthesized …

Biosynthesis of amino deoxy-sugar-conjugated flavonol glycosides by engineered Escherichia coli

RP Pandey, P Parajuli, LL Chu, S Darsandhari… - Biochemical …, 2015 - Elsevier
Abstract Escherichia coli BL21 (DE3) was engineered to divert the flow of carbon flux from
glucose-1-phosphate to thymidine diphosphate 4-keto 4, 6-dideoxy-D-glucose (dTKDG), an …

Toward the production of flavone-7-O-β-D-glucopyranosides using Arabidopsis glycosyltransferase in Escherichia coli

NH Thuan, JW Park, JK Sohng - Process biochemistry, 2013 - Elsevier
Flavonoid glycosides are highly attractive targets due to their dominant roles in clinical,
cosmetic production and in the food industry. In this research, an Escherichia coli strain …

[HTML][HTML] Assembly of a novel biosynthetic pathway for production of the plant flavonoid fisetin in Escherichia coli

SG Stahlhut, S Siedler, S Malla, SJ Harrison… - Metabolic …, 2015 - Elsevier
Plant secondary metabolites are an underutilized pool of bioactive molecules for
applications in the food, pharma and nutritional industries. One such molecule is fisetin …

Donor specificity of YjiC glycosyltransferase determines the conjugation of cytosolic NDP-sugar in in vivo glycosylation reactions

RP Pandey, P Parajuli, RB Gurung, JK Sohng - Enzyme and Microbial …, 2016 - Elsevier
Abstract Escherichia coli BL21 (DE3) was engineered by blocking glucose-1-phosphate
utilizing glucose phosphate isomerase (pgi), glucose-6-phosphate dehydrogenase (zwf) …