Synthetic sugar cassettes for the efficient production of flavonol glycosides in Escherichia coli
P Parajuli, RP Pandey, NTH Trang, AK Chaudhary… - Microbial cell …, 2015 - Springer
Background A multi-monocistronic synthetic vector was used to assemble multiple genes of
a nucleotide diphosphate (NDP)-sugar biosynthetic pathway to construct robust genetic …
a nucleotide diphosphate (NDP)-sugar biosynthetic pathway to construct robust genetic …
Biosynthesis of flavone C-glucosides in engineered Escherichia coli
Two plant-originated C-glucosyltransferases (CGTs) UGT708D1 from Glycine max and
GtUF6CGT1 from Gentiana triflora were accessed for glucosylation of selected flavones …
GtUF6CGT1 from Gentiana triflora were accessed for glucosylation of selected flavones …
Metabolic engineering of Escherichia coli for the biological synthesis of 7-O-xylosyl naringenin
D Simkhada, EM Kim, HC Lee, JK Sohng - Molecules and cells, 2009 - Springer
Flavonoids are a group of polyphenolic compounds that have been recognized as important
due to their physiological and pharmacological roles and their health benefits. Glycosylation …
due to their physiological and pharmacological roles and their health benefits. Glycosylation …
Biosynthesis and production of glycosylated flavonoids in Escherichia coli: current state and perspectives
BG Kim, SM Yang, SY Kim, MN Cha, JH Ahn - Applied Microbiology and …, 2015 - Springer
Flavonoids are plant secondary metabolites containing several hydroxyl groups that are
targets for modification reactions such as methylation and glycosylation. In plants, flavonoids …
targets for modification reactions such as methylation and glycosylation. In plants, flavonoids …
Exploration of glycosylated flavonoids from metabolically engineered E. coli
D Simkhada, NP Kurumbang, HC Lee… - Biotechnology and …, 2010 - Springer
Flavonoids glycosylated with UDP-glucuronic acid and UDP-xylose are spatially distributed
in nature. To produce these glycosides, E. coli was engineered to overexpress biosynthetic …
in nature. To produce these glycosides, E. coli was engineered to overexpress biosynthetic …
Metabolic engineering of Escherichia coli for the biosynthesis of flavonoid-O-glucuronides and flavonoid-O-galactoside
SY Kim, HR Lee, K Park, BG Kim, JH Ahn - Applied microbiology and …, 2015 - Springer
Most flavonoids are glycosylated and the nature of the attached sugar can strongly affect
their physiological properties. Although many flavonoid glycosides have been synthesized …
their physiological properties. Although many flavonoid glycosides have been synthesized …
Biosynthesis of amino deoxy-sugar-conjugated flavonol glycosides by engineered Escherichia coli
Abstract Escherichia coli BL21 (DE3) was engineered to divert the flow of carbon flux from
glucose-1-phosphate to thymidine diphosphate 4-keto 4, 6-dideoxy-D-glucose (dTKDG), an …
glucose-1-phosphate to thymidine diphosphate 4-keto 4, 6-dideoxy-D-glucose (dTKDG), an …
Toward the production of flavone-7-O-β-D-glucopyranosides using Arabidopsis glycosyltransferase in Escherichia coli
Flavonoid glycosides are highly attractive targets due to their dominant roles in clinical,
cosmetic production and in the food industry. In this research, an Escherichia coli strain …
cosmetic production and in the food industry. In this research, an Escherichia coli strain …
[HTML][HTML] Assembly of a novel biosynthetic pathway for production of the plant flavonoid fisetin in Escherichia coli
Plant secondary metabolites are an underutilized pool of bioactive molecules for
applications in the food, pharma and nutritional industries. One such molecule is fisetin …
applications in the food, pharma and nutritional industries. One such molecule is fisetin …
Donor specificity of YjiC glycosyltransferase determines the conjugation of cytosolic NDP-sugar in in vivo glycosylation reactions
Abstract Escherichia coli BL21 (DE3) was engineered by blocking glucose-1-phosphate
utilizing glucose phosphate isomerase (pgi), glucose-6-phosphate dehydrogenase (zwf) …
utilizing glucose phosphate isomerase (pgi), glucose-6-phosphate dehydrogenase (zwf) …
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