Volume regulatory function and sperm membrane dynamics as parameters for evaluating cryoprotective efficiency of a freezing extender
AM Petrunkina, B Gröpper, E Töpfer-Petersen… - Theriogenology, 2005 - Elsevier
In the past years a series of functional assays has been developed to determine the
structural, morphological and functional integrity of the plasma membrane and sperm …
structural, morphological and functional integrity of the plasma membrane and sperm …
Functional significance of the cell volume for detecting sperm membrane changes and predicting freezability in dog semen
AM Petrunkina, B Gropper, AR Gunzel-Apel… - …, 2004 - rep.bioscientifica.com
Due to the similarity of plasma membrane changes induced by capacitation and
cryopreservation, the parameters describing sperm response to capacitating conditions can …
cryopreservation, the parameters describing sperm response to capacitating conditions can …
Osmotic tolerance limits and effects of cryoprotectants on the motility, plasma membrane integrity and acrosomal integrity of rat sperm
Osmotic stress is an important factor that can result in cell damage during cryopreservation.
The objectives of this study were to determine:(1) isosmotic sperm cell volume;(2) …
The objectives of this study were to determine:(1) isosmotic sperm cell volume;(2) …
Membrane changes during different stages of a freeze–thaw protocol for equine semen cryopreservation
DM Neild, BM Gadella, MG Chaves, MH Miragaya… - Theriogenology, 2003 - Elsevier
Many theories have been postulated concerning the possible effects of cryopreservation on
spermatozoa, including suggestions the freeze–thawing process produces membranes that …
spermatozoa, including suggestions the freeze–thawing process produces membranes that …
Capacitation-like changes in equine spermatozoa following cryopreservation
The primary objective of this study was to assess plasma membrane characteristics and
activation of signal transduction pathways in equine spermatozoa during both in vitro …
activation of signal transduction pathways in equine spermatozoa during both in vitro …
Effects of temperature and restoration of osmotic equilibrium during thawing on the induction of plasma membrane damage in cryopreserved ram spermatozoa
WV Holt, RD North - Biology of Reproduction, 1994 - academic.oup.com
The objective of this investigation was to examine the nature of freeze/thaw-induced plasma
membrane damage in an effort to validate hypotheses about cryoinjury in ram spermatozoa …
membrane damage in an effort to validate hypotheses about cryoinjury in ram spermatozoa …
[PDF][PDF] The tonicity of murine epididymal spermatozoa and their permeability towards common cryoprotectants and epididymal osmolytes
TG Cooper, JP Barfield, CH Yeung - Reproduction, 2008 - researchgate.net
The permeability of murine cauda epididymidal spermatozoa was determined from the
swelling caused by penetrating agents at isotonicity, which lies between 422 and 530 …
swelling caused by penetrating agents at isotonicity, which lies between 422 and 530 …
Osmotic stress and membrane phase changes during freezing of stallion sperm: mode of action of cryoprotective agents
H Oldenhof, M Gojowsky, S Wang, S Henke… - Biology of …, 2013 - academic.oup.com
The aim of this study was to determine how different membrane-permeable and-
impermeable cryoprotective agents modulate tolerance of stallion sperm to osmotic stress …
impermeable cryoprotective agents modulate tolerance of stallion sperm to osmotic stress …
Osmotic characteristics of mouse spermatozoa in the presence of extenders and sugars
Y Agca, J Gilmore, M Byers, EJ Woods… - Biology of …, 2002 - academic.oup.com
Successful cryopreservation requires cells to tolerate volume excursions experienced during
permeating cryoprotectant equilibration and during cooling and warming. However, prior …
permeating cryoprotectant equilibration and during cooling and warming. However, prior …
Membrane fluidity predicts the outcome of cryopreservation of human spermatozoa
MN Giraud, C Motta, D Boucher… - Human …, 2000 - academic.oup.com
Semen cryopreservation is an important procedure in the treatment of human infertility.
However, the ability of spermatozoa to survive freeze/thaw processes varies between …
However, the ability of spermatozoa to survive freeze/thaw processes varies between …
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