Volume regulatory function and sperm membrane dynamics as parameters for evaluating cryoprotective efficiency of a freezing extender

AM Petrunkina, B Gröpper, E Töpfer-Petersen… - Theriogenology, 2005 - Elsevier
In the past years a series of functional assays has been developed to determine the
structural, morphological and functional integrity of the plasma membrane and sperm …

Functional significance of the cell volume for detecting sperm membrane changes and predicting freezability in dog semen

AM Petrunkina, B Gropper, AR Gunzel-Apel… - …, 2004 - rep.bioscientifica.com
Due to the similarity of plasma membrane changes induced by capacitation and
cryopreservation, the parameters describing sperm response to capacitating conditions can …

Osmotic tolerance limits and effects of cryoprotectants on the motility, plasma membrane integrity and acrosomal integrity of rat sperm

W Si, JD Benson, H Men, JK Critser - Cryobiology, 2006 - Elsevier
Osmotic stress is an important factor that can result in cell damage during cryopreservation.
The objectives of this study were to determine:(1) isosmotic sperm cell volume;(2) …

Membrane changes during different stages of a freeze–thaw protocol for equine semen cryopreservation

DM Neild, BM Gadella, MG Chaves, MH Miragaya… - Theriogenology, 2003 - Elsevier
Many theories have been postulated concerning the possible effects of cryopreservation on
spermatozoa, including suggestions the freeze–thawing process produces membranes that …

Capacitation-like changes in equine spermatozoa following cryopreservation

AD Thomas, SA Meyers, BA Ball - Theriogenology, 2006 - Elsevier
The primary objective of this study was to assess plasma membrane characteristics and
activation of signal transduction pathways in equine spermatozoa during both in vitro …

Effects of temperature and restoration of osmotic equilibrium during thawing on the induction of plasma membrane damage in cryopreserved ram spermatozoa

WV Holt, RD North - Biology of Reproduction, 1994 - academic.oup.com
The objective of this investigation was to examine the nature of freeze/thaw-induced plasma
membrane damage in an effort to validate hypotheses about cryoinjury in ram spermatozoa …

[PDF][PDF] The tonicity of murine epididymal spermatozoa and their permeability towards common cryoprotectants and epididymal osmolytes

TG Cooper, JP Barfield, CH Yeung - Reproduction, 2008 - researchgate.net
The permeability of murine cauda epididymidal spermatozoa was determined from the
swelling caused by penetrating agents at isotonicity, which lies between 422 and 530 …

Osmotic stress and membrane phase changes during freezing of stallion sperm: mode of action of cryoprotective agents

H Oldenhof, M Gojowsky, S Wang, S Henke… - Biology of …, 2013 - academic.oup.com
The aim of this study was to determine how different membrane-permeable and-
impermeable cryoprotective agents modulate tolerance of stallion sperm to osmotic stress …

Osmotic characteristics of mouse spermatozoa in the presence of extenders and sugars

Y Agca, J Gilmore, M Byers, EJ Woods… - Biology of …, 2002 - academic.oup.com
Successful cryopreservation requires cells to tolerate volume excursions experienced during
permeating cryoprotectant equilibration and during cooling and warming. However, prior …

Membrane fluidity predicts the outcome of cryopreservation of human spermatozoa

MN Giraud, C Motta, D Boucher… - Human …, 2000 - academic.oup.com
Semen cryopreservation is an important procedure in the treatment of human infertility.
However, the ability of spermatozoa to survive freeze/thaw processes varies between …