Transcription and DNA supercoiling are known to be linked by a cause‐effect relationship
that operates in both directions. It is proposed here that this two‐way relationship may be …

It is well known that open complex formation between promoters and RNA polymerase is
thermodynamically favored by negative su-percoiling of the DNA template. The effects of …

The translocation of an RNA polymerase elongation complex along double helical DNA has
been proposed to generate positive supercoiling waves ahead of and negative supercoiling …

DNA supercoiling is known to influence the pattern of gene expression in prokaryotes. Thus
the mechanism of transcription initiation and the topological state of the template are …

Transcription by RNA polymerase can stimulate localized DNA supercoiling in Escherichia
coli. In vivo, there is extensive experimental support for a “twin-domain” model in which …

Transcription of a right-handed double-helical DNA requires a relative rotation of the RNA
polymerase and its nascent RNA around the DNA. We describe conditions under which the …

The inactive prokaryotic leu-500 promoter (P leu-500) contains a single A-to-G point
mutation in the− 10 region of the leucine operon promoter, which causes leucine …

According to the twin‐supercoiled domain model, a local domain with a high level of DNA
supercoiling can be generated between two divergently transcribed promoters. We have …

The scale of negative DNA supercoiling generated by transcription in Top+ Escherichia coli
cells was assessed from the efficiency of cruciform formation upstream of a regulated …

In both eukaryotes and prokaryotes, negative supercoiling of chromosomal DNA acts locally
to regulate a variety of cellular processes, such as transcription, replication, recombination …