We evaluated topological predictions for nine different programs, HMMTOP, TMHMM,
SVMTOP, DAS, SOSUI, TOPCONS, PHOBIUS, MEMSAT-SVM (hereinafter referred to as …

Limited experimental data may be very useful to discriminate between membrane topology
models of membrane proteins derived from different methods. A membrane topology …

We selected 10 transmembrane (TM) prediction methods (KKD, TMpred, TopPred II, DAS,
TMAP, MEMSAT 2, SOSUI, PRED-TMR2, TMHMM 2.0 and HMMTOP 2.0) and re-assessed …

ExTopoDB is a publicly accessible database of experimentally derived topological models of
transmembrane proteins. It contains information collected from studies in the literature that …

α-Helical transmembrane proteins termed membrane transporters mediate the passage of
small hydrophilic substrate molecules across biological lipid bilayer membranes. Annotating …

A new method is described for prediction of protein membrane topology (intra‐and
extracellular sidedness) from multiply aligned amino acid sequences after determination of …

How reliable and useful are predictions of transmembrane segments (TMSs) of membrane
proteins from the amino acid sequences? It remains still under debate. Kyte and Doolittle …

Membrane protein topology predictions can be markedly improved by the inclusion of even
very limited experimental information. We have recently introduced an approach for the …

Three-dimensional structures have been elucidated for very few integral membrane
proteins. Computer methods can be used as guides for estimation of solute transport protein …

We show that the so‐called 'positive inside'rule, ie the observation that positively charged
amino acids tend to be more prevalent in cytoplasmic than in extra‐cytoplasmic segments in …