Two new protein conjugates were prepared and studied to develop and compare two (direct
and indirect) competitive enzyme-linked immunosorbent assay (ELISA) formats for the …

Homogeneous assays are attractive because they are performed in only one phase, namely,
the liquid phase, and thus, they do not require separation of phases as their heterogeneous …

Non-invasive measurement of cortisol in saliva is of prime importance as it represents a
bioavailable neuroendocrine marker for stress. Therefore, in this study, we developed an …

A general method of performing non-competitive immunoassays for a low-molecular-mass
analyte was developed and applied to cortisol determination in saliva samples. The method …

Cortisol 3-(o-carboxymethyl) oxime (C3-CMO) and a commercially available biotin-
hydrazide derivative were used to synthesize a C3-CMO-biotin conjugate. C3-CMO was …

The analysis of hormones in saliva is a powerful tool in the assessment of a patient's
endocrine function, since it allows multiple noninvasive samplings. Since salivary levels of …

The monitoring of salivary cortisol as a key biomarker of an individual's stress response has
been increasingly focused on. This paper describes the development of a novel cortisol …

Cortisol is a steroidal hormone and an important stress marker. Free serum cortisol
concentration has been identified to correlate well with free salivary cortisol. In this present …

The aim of this work was to develop a method for determination of cortisol in saliva by liquid
chromatography–tandem mass spectrometry (LC–MS–MS). Saliva was sampled on …

Measurement of cortisol levels in body fluids is important for monitoring pituitary gland and
adrenal functions. To develop a specific and standardized enzyme-linked immunosorbent …