[HTML][HTML] Base editors for citrus gene editing

X Huang, Y Wang, N Wang - Frontiers in Genome Editing, 2022 - frontiersin.org
Base editors, such as adenine base editors (ABE) and cytosine base editors (CBE), provide
alternatives for precise genome editing without generating double-strand breaks (DSBs) …

Development of multiplex genome editing toolkits for citrus with high efficacy in biallelic and homozygous mutations

X Huang, Y Wang, J Xu, N Wang - Plant molecular biology, 2020 - Springer
Key message We have developed multiplex genome editing toolkits for citrus that
significantly improve citrus genome editing efficacy. Abstract CRISPR/Cas systems have …

Genome editing in citrus tree with CRISPR/Cas9

H Jia, X Zou, V Orbovic, N Wang - Plant Genome Editing with CRISPR …, 2019 - Springer
CRISPR/Cas9 has been widely employed to edit genome in most of the organisms,
including animal, plant, fungus, and microbe. Here we describe the modification of citrus …

CRISPR‐LbCas12a‐mediated modification of citrus

H Jia, V Orbović, N Wang - Plant biotechnology journal, 2019 - Wiley Online Library
Summary Recently, CRISPR‐Cas12a (Cpf1) from Prevotella and Francisella was
engineered to modify plant genomes. In this report, we employed CRISPR‐LbCas12a …

[HTML][HTML] Efficient CRISPR/Cas9 genome editing with Citrus embryogenic cell cultures

M Dutt, Z Mou, X Zhang, SE Tanwir, JW Grosser - BMC biotechnology, 2020 - Springer
Background Development of precise genome editing strategies is a prerequisite for
producing edited plants that can aid in the study of gene function and help understand the …

Efficient base editing in tomato using a highly expressed transient system

S Yuan, S Kawasaki, IMY Abdellatif, K Nishida… - Plant Cell Reports, 2021 - Springer
Key message Base editing in tomatoes was achieved by transient expression. Abstract The
Solanaceae plants, particularly the tomato (Solanum lycopersicum), is of huge economic …

[HTML][HTML] Transgene-Free Genome Editing in Tomato and Potato Plants Using Agrobacterium-Mediated Delivery of a CRISPR/Cas9 Cytidine Base Editor

F Veillet, L Perrot, L Chauvin, MP Kermarrec… - International Journal of …, 2019 - mdpi.com
Genome editing tools have rapidly been adopted by plant scientists for gene function
discovery and crop improvement. The current technical challenge is to efficiently induce …

[HTML][HTML] Highly efficient generation of canker-resistant sweet orange enabled by an improved CRISPR/Cas9 system

X Huang, Y Wang, N Wang - Frontiers in Plant Science, 2022 - frontiersin.org
Sweet orange (Citrus sinensis) is the most economically important species for the citrus
industry. However, it is susceptible to many diseases including citrus bacterial canker …

Rapid and efficient CRISPR/Cas9 gene editing in Citrus using the YAO promoter

F Zhang, C LeBlanc, VF Irish, Y Jacob - Plant Cell Reports, 2017 - Springer
Citrus is one of the most important fruit crops in the world. Global commercial orange (Citrus
sinensis) production exceeded 47 million tons (mt) in 2015 (https://apps. fas. usda …

Genome editing of the disease susceptibility gene CsLOB1 in citrus confers resistance to citrus canker

H Jia, Y Zhang, V Orbović, J Xu… - Plant biotechnology …, 2017 - Wiley Online Library
Citrus is a highly valued tree crop worldwide, while, at the same time, citrus production faces
many biotic challenges, including bacterial canker and Huanglongbing (HLB). Breeding for …