Many reference genes are used by different laboratories for gene expression analyses to
indicate the relative amount of input RNA/DNA in the experiment. These reference genes …

It has been shown that genes considered to be valid reference genes using semi-
quantitative techniques (eg northern blot) appear to be less reliable when highly sensitive …

The transcript pool of a plant part, under any given condition, is a collection of mRNAs that
will pave the way for a biochemical reaction of the plant to stimuli. Over the past decades …

Plants are exposed to variety of stress factors (biotic and abiotic) in both natural and
agricultural conditions, which have a serious impact on plant development and growth …

Real-time quantitative reverse transcription PCR (RT-qPCR) is a rapid and reliable method
for gene expression studies. Normalization based on reference genes can increase the …

Plants are considered the backbone of life on earth. The colorful life on this planet has
emerged as a consequence of over 3.5 billion years of unceasing evolution. Life on earth …

Quantitative PCR (qPCR) is a powerful tool for measuring gene expression levels. Accurate
and reproducible results are dependent on the correct choice of reference genes for data …

Pearl millet [Pennisetum glaucum (L.) R. Br.] a widely used grain and forage crop, is grown
in areas frequented with one or more abiotic stresses, has superior drought and heat …

Thus far conventional plant breeding methods have been successfully used for sustainable
food production worldwide. Human population is increasing at an alarming rate in …

Abiotic stresses such as high temperature, low temperature, drought, flooding, salinity,
heavy metals, radiation, ozone are the most threatening and limiting factors for agricultural …