Construct design for efficient, effective and high‐throughput gene silencing in plants
Post‐transcriptional silencing of plant genes using anti‐sense or co‐suppression constructs
usually results in only a modest proportion of silenced individuals. Recent work has …
usually results in only a modest proportion of silenced individuals. Recent work has …
High-throughput vectors for efficient gene silencing in plants
CA Helliwell, SV Wesley, AJ Wielopolska… - Functional plant …, 2002 - CSIRO Publishing
A major challenge in the post-genome era of plant biology is to determine the functions of all
genes in the plant genome. A straightforward approach to this problem is to reduce or …
genes in the plant genome. A straightforward approach to this problem is to reduce or …
hpRNA-Mediated Targeting of the Arabidopsis FAD2 Gene Gives Highly Efficient and Stable Silencing
The endogenous Δ12-desaturase gene (FAD2) in Arabidopsis was targeted for silencing
using seed-specific cosuppression (CS), hairpin (HP) RNA (hpRNA), and intron-spliced HP …
using seed-specific cosuppression (CS), hairpin (HP) RNA (hpRNA), and intron-spliced HP …
Constructs and methods for high-throughput gene silencing in plants
C Helliwell, P Waterhouse - Methods, 2003 - Elsevier
Gene silencing can be achieved by transformation of plants with constructs that express self-
complementary (termed hairpin) RNA containing sequences homologous to the target …
complementary (termed hairpin) RNA containing sequences homologous to the target …
Ectopic pairing of homologous DNA and post-transcriptional gene silencing in transgenic plants
DC Baulcombe, JJ English - Current Opinion in Biotechnology, 1996 - Elsevier
Recent studies indicate that co-suppression and other types of post-transcriptional gene
silencing in plants are initiated by homology-dependent pairing of transgene sequences …
silencing in plants are initiated by homology-dependent pairing of transgene sequences …
Exploring plant genomes by RNA-induced gene silencing
PM Waterhouse, CA Helliwell - Nature Reviews Genetics, 2003 - nature.com
The nucleotide sequences of several animal, plant and bacterial genomes are now known,
but the functions of many of the proteins that they are predicted to encode remain unclear …
but the functions of many of the proteins that they are predicted to encode remain unclear …
Temporal and spatial control of gene silencing in transgenic plants by inducible expression of double‐stranded RNA
Downregulation of endogenous genes via post‐transcriptional gene silencing (PTGS) is a
key to the characterization of gene function in plants. The recent discovery that double …
key to the characterization of gene function in plants. The recent discovery that double …
RNAi for functional genomics in plants
KM McGinnis - Briefings in functional genomics, 2010 - academic.oup.com
RNAi refers to several different types of gene silencing mediated by small, dsRNA
molecules. Over the course of 20 years, the scientific understanding of RNAi has developed …
molecules. Over the course of 20 years, the scientific understanding of RNAi has developed …
A chemical‐regulated inducible RNAi system in plants
Constitutive expression of an intron‐containing self‐complementary 'hairpin'RNA (ihpRNA)
has recently been shown to efficiently silence target genes in transgenic plants. However …
has recently been shown to efficiently silence target genes in transgenic plants. However …
Engineering novel traits in plants through RNA interference
RNA interference (RNAi) is a homology-dependent gene silencing technology that involves
double-stranded RNA directed against a target gene or its promoter region. Using hairpin …
double-stranded RNA directed against a target gene or its promoter region. Using hairpin …