Engineered chloroplast dsRNA silences cytochrome p450 monooxygenase, V‐ATPase and chitin synthase genes in the insect gut and disrupts Helicoverpa armigera …

S Jin, ND Singh, L Li, X Zhang… - Plant biotechnology …, 2015 - Wiley Online Library
In the past two decades, chloroplast genetic engineering has been advanced to achieve
high‐level protein accumulation but not for down‐regulation of targeted genes. Therefore, in …

In-Plant Protection against Helicoverpa armigera by Production of Long hpRNA in Chloroplasts

J Bally, GJ McIntyre, RL Doran, K Lee, A Perez… - Frontiers in plant …, 2016 - frontiersin.org
Expressing double-stranded RNA (dsRNA) in transgenic plants to silence essential genes
within herbivorous pests is referred to as trans-kingdom RNA interference (TK-RNAi) and …

RNA Interference in the Tobacco Hornworm, Manduca sexta, Using Plastid-Encoded Long Double-Stranded RNA

WG Burke, E Kaplanoglu, I Kolotilin… - Frontiers in plant …, 2019 - frontiersin.org
RNA interference (RNAi) is a promising method for controlling pest insects by silencing the
expression of vital insect genes to interfere with development and physiology; however …

Length-dependent accumulation of double-stranded RNAs in plastids affects RNA interference efficiency in the Colorado potato beetle

W He, W Xu, L Xu, K Fu, W Guo, R Bock… - Journal of …, 2020 - academic.oup.com
Transplastomic potato plants expressing double-stranded RNA (dsRNA) targeted against
essential genes of the Colorado potato beetle (CPB) can be lethal to larvae by triggering an …

Control of a sap-sucking insect pest by plastid-mediated RNA interference

Y Dong, M Wu, Q Zhang, J Fu, FV Loiacono, Y Yang… - Molecular Plant, 2022 - cell.com
Expression of double-stranded RNAs in plastids offers great potential for the efficient control
of chewing insects. However, many insect pests do not consume plant tissue but rather feed …

Improved insect‐proofing: expressing double‐stranded RNA in chloroplasts

J Bally, E Fishilevich, AJ Bowling… - Pest management …, 2018 - Wiley Online Library
RNA interference (RNAi) was discovered almost 20 years ago and has been exploited
worldwide to silence genes in plants and animals. A decade later, it was found that …

Inaccessibility to double‐stranded RNAs in plastids restricts RNA interference in Bemisia tabaci (whitefly)

Y Dong, Y Yang, Z Wang, M Wu, J Fu… - Pest management …, 2020 - Wiley Online Library
Background RNA interference (RNAi) has emerged as a promising technology for insect
pest control. Because of the accumulation of high levels of long double‐stranded RNAs …

Double-stranded RNA oral delivery methods to induce RNA interference in phloem and plant-sap-feeding hemipteran insects

SKB Ghosh, WB Hunter, AL Park… - JoVE (Journal of …, 2018 - jove.com
Phloem and plant sap feeding insects invade the integrity of crops and fruits to retrieve
nutrients, in the process damaging food crops. Hemipteran insects account for a number of …

Next-generation insect-resistant plants: RNAi-mediated crop protection

J Zhang, SA Khan, DG Heckel, R Bock - Trends in biotechnology, 2017 - cell.com
Plant-mediated RNA interference (RNAi) shows great potential in crop protection. It relies on
plants stably expressing double-stranded RNAs (dsRNAs) that target essential genes in pest …

Efficient control of western flower thrips by plastid-mediated RNA interference

M Wu, Y Dong, Q Zhang, S Li… - Proceedings of the …, 2022 - National Acad Sciences
Plastid-mediated RNA interference (PM-RNAi) has emerged as a promising strategy for pest
control. Expression from the plastid genome of stable double-stranded RNAs (dsRNAs) …