The membrane potential (ΔΨ) and the pH gradient (ΔpH) across the membrane of the
insulin-secretory granule were determined in studies in vitro from the uptake of the permeant …

The concentrations of Zn2+, Ca2+, Mg2+, Pi and adenine nucleotides were determined in
insulin-secretory granules prepared from a transplantable rat insulinoma. Differential and …

Insulin-secretory granules isolated from a pancreatic islet-cell tumour by centrifugation on
Percoll density gradients exhibited a membrane-associated Mg2+-dependent ATPase …

β-Granules were prepared from micro-dissected pancreatic islets of obese–hyperglycaemic
mice. This fraction contained 60% of the insulin, 30% of the cytochrome oxidase, 16% of the …

Glucose-induced insulin secretion requires a rise in β-cell cytosolic Ca 2+([Ca 2+] c) that
triggers exocytosis and a mechanistically unexplained amplification of the action of [Ca 2+] …

Density gradient centrifugation techniques, using iso-osmotic colloidal silica suspensions
(Percoll), were developed for the isolation of insulin secretory granules from a transplantable …

A partially purified secretory granule fraction, isolated from rat islets of Langerhans by
differential centrifugation, was used for investigating the stability of the beta granules during …

The release of insulin evoked by nutrient secreta-gogues (eg, glucose) in the pancreatic B-
cell is mediated by a sequence of metabolic, ionic, and motile events, culminating in the …

Secretory granule-enriched fractions prepared from isolated rat islets of Langerhans,
previously labeled in culture for 18 h with [3H] leucine, have been lysed and separated into …

1. An in vitro system is described in which insulin beta‐granule‐plasma membrane
interaction can be studied. 2. The system shows an absolute requirement for physiological …