Recently, the cytosine modifications 5-hydroxymethylcytosine (5hmC) and 5-formylcytosine
(5fC) were found to exist in the genomic deoxyribonucleic acid (DNA) of a wide range of …

To uncover the function of and interplay between the mammalian cytosine modifications 5-
methylcytosine (5mC) and 5-hydroxymethylcytosine (5hmC), new techniques and advances …

Active DNA demethylation in mammals involves oxidation of 5-methylcytosine (5mC) to 5-
hydroxymethylcytosine (5hmC), 5-formylcytosine (5fC) and 5-carboxylcytosine (5caC) …

Summary The study of 5-hydroxylmethylcytosines (5hmC) has been hampered by the lack of
a method to map it at single-base resolution on a genome-wide scale. Affinity purification …

A complete understanding of the potential function of 5-hydroxymethylcytosine (5-hmC), a
DNA cytosine modification in mammalian cells, requires an accurate single-base resolution …

Summary Mapping genome-wide 5-hydroxymethylcytosine (5hmC) and 5-formylcytosine
(5fC) at single-base resolution is important to understand their biological functions. We …

5-Methylcytosine can be converted to 5-hydroxymethylcytosine (5hmC) in mammalian DNA
by the ten-eleven translocation (TET) enzymes. We introduce oxidative bisulfite sequencing …

Bisulfite sequencing (BS-seq) to detect 5-methylcytosine (5mC) is limited by lengthy reaction
times, severe DNA damage, overestimation of 5mC level and incomplete C-to-U conversion …

Formylcytosine (5fC) is a rare base found in mammalian DNA and thought to be involved in
active DNA demethylation. Here, we show that developmental dynamics of 5fC levels in …

methylcytosine (5mC) is the most important DNA modification in mammalian genomes. The
ideal method for 5mC localization would be both nondestructive of DNA and direct, without …