Background Testing an ever-increasing number of CRISPR components is challenging
when developing new genome engineering tools. Plant biotechnology has few high …

Targeted genome-editing technology using designed nucleases has been rapidly
advancing and 17 catalyzing significant breakthroughs in the life science. Custom-designed …

CRISPR-Cas9 enabled genome engineering has great potential for improving agriculture
productivity, but the possibility of unintended off-target edits has evoked some concerns …

Many Cas9-derived base editors have been developed for precise C–to–T and A–to–G base
editing in plants (Molla et al., 2021). They are typically based on a SpCas9 nickase or its …

Background CRISPR-Cas12a (formerly Cpf1) is an RNA-guided endonuclease with distinct
features that have expanded genome editing capabilities. Cas12a-mediated genome editing …

The development of CRISPR‐based editors recognizing distinct protospacer‐adjacent
motifs (PAMs), or having different spacer length/structure requirements broadens the range …

CRISPR/Cas9-based systems are efficient genome editing tools in a variety of plant species
including soybean. Most of the gene edits in soybean plants are somatic and non …

Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) mediated genome
editing is a powerful approach for crop augmentations. Traditional transformation methods …

Conventional breeding techniques for crop improvement are based on hybridization and
selection. However, due to the long breeding cycles of crops and the potentially …

CRISPR-Cas9,-Cas12a,-Cas12b, and-Cas13 have been harnessed for genome
engineering in human and plant cells (Liu et al., 2022). However, the large size of these Cas …