Time-resolved single-cell RNA-seq using metabolic RNA labelling

F Erhard, AE Saliba, A Lusser, C Toussaint… - Nature Reviews …, 2022 - nature.com
Single-cell RNA genomics technologies are revolutionizing biomedical science by profiling
single cells with unprecedented resolution, providing fundamental insights into the role of …

User-friendly, scalable tools and workflows for single-cell RNA-seq analysis

P Moreno, N Huang, JR Manning, S Mohammed… - Nature …, 2021 - nature.com
To the Editor—As single-cell RNA sequencing (scRNA-seq) becomes widespread,
accessible and scalable computational pipelines for data analysis are needed. We introduce …

[HTML][HTML] Biological and medical importance of cellular heterogeneity deciphered by single-cell RNA sequencing

RK Gupta, J Kuznicki - Cells, 2020 - mdpi.com
The present review discusses recent progress in single-cell RNA sequencing (scRNA-seq),
which can describe cellular heterogeneity in various organs, bodily fluids, and pathologies …

[HTML][HTML] Methods, challenges and potentials of single cell RNA-seq

D Hebenstreit - Biology, 2012 - mdpi.com
RNA-sequencing (RNA-seq) has become the tool of choice for transcriptomics. Several
recent studies demonstrate its successful adaption to single cell analysis. This allows new …

[HTML][HTML] Single-cell RNA sequencing and its combination with protein and DNA analyses

JR Choi, KW Yong, JY Choi, AC Cowie - Cells, 2020 - mdpi.com
Heterogeneity in cell populations poses a significant challenge for understanding complex
cell biological processes. The analysis of cells at the single-cell level, especially single-cell …

[HTML][HTML] Exploring the single-cell RNA-seq analysis landscape with the scRNA-tools database

L Zappia, B Phipson, A Oshlack - PLoS computational biology, 2018 - journals.plos.org
As single-cell RNA-sequencing (scRNA-seq) datasets have become more widespread the
number of tools designed to analyse these data has dramatically increased. Navigating the …

Single-cell RNA sequencing analysis: a step-by-step overview

S Slovin, A Carissimo, F Panariello, A Grimaldi… - RNA bioinformatics, 2021 - Springer
Thanks to innovative sample-preparation and sequencing technologies, gene expression in
individual cells can now be measured for thousands of cells in a single experiment. Since its …

Single‐cell RNA sequencing technologies and applications: A brief overview

D Jovic, X Liang, H Zeng, L Lin, F Xu… - Clinical and …, 2022 - Wiley Online Library
Single‐cell RNA sequencing (scRNA‐seq) technology has become the state‐of‐the‐art
approach for unravelling the heterogeneity and complexity of RNA transcripts within …

Entering the era of single-cell transcriptomics in biology and medicine

R Sandberg - Nature methods, 2014 - nature.com
Recent technical advances have enabled RNA sequencing (RNA-seq) in single cells.
Exploratory studies have already led to insights into the dynamics of differentiation, cellular …

Multiplexed single-cell RNA-seq via transient barcoding for simultaneous expression profiling of various drug perturbations

D Shin, W Lee, JH Lee, D Bang - Science advances, 2019 - science.org
The development of high-throughput single-cell RNA sequencing (scRNA-seq) has enabled
access to information about gene expression in individual cells and insights into new …