An overview of epigenetic assays

JT DeAngelis, WJ Farrington, TO Tollefsbol - Molecular biotechnology, 2008 - Springer
A significant portion of ongoing epigenetic research involves the investigation of DNA
methylation and chromatin modification patterns seen throughout many biological …

Combined chromatin immunoprecipitation and bisulfite methylation sequencing analysis

Y Li, TO Tollefsbol - Epigenetics Protocols, 2011 - Springer
Epigenetic mechanisms control gene transcription primarily through regulating chromatin
structures and DNA methylation. Transcription factors can also affect gene transcription …

Genome‐wide DNA methylation profiling

M Bibikova, JB Fan - Wiley Interdisciplinary Reviews: Systems …, 2010 - Wiley Online Library
DNA methylation plays a critical role in the regulation of gene expression. The ability to
access the methylation status for a large number of genes or the entire genome should …

In vivo analysis of DNA methylation patterns recognized by specific proteins: coupling CHIP and bisulfite analysis

MR Matarazzo, F Lembo, T Angrisano, E Ballestar… - …, 2004 - Taylor & Francis
The three-way connection between DNA methylation, chromatin configuration, and
transcriptional regulation is under increasing attention, but the fine rules governing the epi …

[HTML][HTML] Microplate-based platform for combined chromatin and DNA methylation immunoprecipitation assays

J Yu, Q Feng, Y Ruan, R Komers, N Kiviat… - BMC Molecular …, 2011 - Springer
Background The processes that compose expression of a given gene are far more complex
than previously thought presenting unprecedented conceptual and mechanistic challenges …

[HTML][HTML] Laboratory methods to decipher epigenetic signatures: a comparative review

R Halabian, V Arshad, A Ahmadi, P Saeedi… - Cellular & Molecular …, 2021 - Springer
Epigenetics refers to nucleotide sequence-independent events, and heritable changes,
including DNA methylation and histone modification (as the two main processes) …

Advances in epigenetic technology

TO Tollefsbol - Epigenetics protocols, 2011 - Springer
Epigenetics refers to the collective heritable changes in phenotype that arise independent of
genotype. Two broad areas of epigenetics are DNA methylation and histone modifications …

Epigenetic analysis: ChIP-chip and ChIP-seq

M Pellegrini, R Ferrari - Next Generation Microarray Bioinformatics …, 2012 - Springer
The access of transcription factors and the replication machinery to DNA is regulated by the
epigenetic state of chromatin. In eukaryotes, this complex layer of regulatory processes …

[HTML][HTML] DNA methylation: a timeline of methods and applications

A Harrison, A Parle-McDermott - Frontiers in genetics, 2011 - frontiersin.org
DNA methylation is a biochemical process where a DNA base, usually cytosine, is
enzymatically methylated at the 5-carbon position. An epigenetic modification associated …

Introduction to epigenomics and epigenome-wide analysis

MJ Fazzari, JM Greally - Statistical Methods in Molecular Biology, 2010 - Springer
Epigenetics is the study of heritable change other than those encoded in DNA sequence.
Cytosine methylation of DNA at CpG dinucleotides is the most well-studied epigenetic …