Bacterial chemotaxis in static gradients quantified in a biopolymer membrane-integrated microfluidic platform

P Hu, KL Ly, LPH Pham, AE Pottash, K Sheridan… - Lab on a Chip, 2022 - pubs.rsc.org
Chemotaxis is a fundamental bacterial response mechanism to changes in chemical
gradients of specific molecules known as chemoattractant or chemorepellent. The …

Microfluidics for bacterial chemotaxis

T Ahmed, TS Shimizu, R Stocker - Integrative biology, 2010 - academic.oup.com
Microfluidics is revolutionizing the way we study the motile behavior of cells, by enabling
observations at high spatial and temporal resolution in carefully controlled …

Multiplexed microfluidic screening of bacterial chemotaxis

MR Stehnach, RJ Henshaw, SA Floge, JS Guasto - Elife, 2023 - elifesciences.org
Microorganism sensing of and responding to ambient chemical gradients regulates a myriad
of microbial processes that are fundamental to ecosystem function and human health and …

[HTML][HTML] A simple and reusable bilayer membrane-based microfluidic device for the study of gradient-mediated bacterial behaviors

W Shang, CY Tsao, X Luo, M Teodoro, R McKay… - …, 2017 - pubs.aip.org
We have developed a user-friendly microfluidic device for the study of gradient-mediated
bacterial behaviors, including chemotaxis. This device rapidly establishes linear …

Investigation of bacterial chemotaxis using a simple three-point microfluidic system

H Kim, J Ali, K Phuyal, S Park, MJ Kim - BioChip Journal, 2015 - Springer
A three-point microfluidic system was developed and used to experimentally verify bacterial
chemotaxis with known chemoeffectors. Using pneumatically-controlled micro-valves, the …

A microfluidic device for quantifying bacterial chemotaxis in stable concentration gradients

DL Englert, MD Manson, A Jayaraman - JoVE (Journal of Visualized …, 2010 - jove.com
Chemotaxis allows bacteria to approach sources of attractant chemicals or to avoid sources
of repellent chemicals. Bacteria constantly monitor the concentration of specific …

Bacterial chemotaxis in linear and nonlinear steady microfluidic gradients

T Ahmed, TS Shimizu, R Stocker - Nano letters, 2010 - ACS Publications
Diffusion-based microfluidic devices can generate steady, arbitrarily shaped chemical
gradients without requiring fluid flow and are ideal for studying chemotaxis of free-swimming …

Investigation of bacterial chemotaxis in flow-based microfluidic devices

DL Englert, MD Manson, A Jayaraman - Nature protocols, 2010 - nature.com
The plug-in-pond and capillary assays are convenient methods for measuring attractant and
repellent bacterial chemotaxis. However, these assays do not provide quantitative …

Pump-less static microfluidic device for analysis of chemotaxis of Pseudomonas aeruginosa using wetting and capillary action

HH Jeong, SH Lee, CS Lee - Biosensors and Bioelectronics, 2013 - Elsevier
Bacterial chemotaxis is a complex response to temporal changes of concentrations of
chemoeffectors. Conventional methods, including microfluidic approaches, for the precise …

Microchannel-nanopore device for bacterial chemotaxis assays

ML Kovarik, PJB Brown, DT Kysela, C Berne… - Analytical …, 2010 - ACS Publications
Motile bacteria bias the random walk of their motion in response to chemical gradients by
the process termed chemotaxis, which allows cells to accumulate in favorable environments …