Next generation sequencing approaches to evaluate water and wastewater quality

E Garner, BC Davis, E Milligan, MF Blair, I Keenum… - Water Research, 2021 - Elsevier
The emergence of next generation sequencing (NGS) is revolutionizing the potential to
address complex microbiological challenges in the water industry. NGS technologies can …

[HTML][HTML] Next-generation sequencing (NGS) for assessment of microbial water quality: current progress, challenges, and future opportunities

BF Tan, C Ng, JP Nshimyimana, LL Loh… - Frontiers in …, 2015 - frontiersin.org
Water quality is an emergent property of a complex system comprised of interacting
microbial populations and introduced microbial and chemical contaminants. Studies …

Potential applications of next generation DNA sequencing of 16S rRNA gene amplicons in microbial water quality monitoring

J Vierheilig, D Savio, RE Ley, RL Mach… - Water Science and …, 2015 - iwaponline.com
The applicability of next generation DNA sequencing (NGS) methods for water quality
assessment has so far not been broadly investigated. This study set out to evaluate the …

[HTML][HTML] Molecular methods for pathogenic bacteria detection and recent advances in wastewater analysis

S Zhang, X Li, J Wu, L Coin, J O'brien, F Hai, G Jiang - Water, 2021 - mdpi.com
With increasing concerns about public health and the development of molecular techniques,
new detection tools and the combination of existing approaches have increased the abilities …

Application of high-throughput sequencing technologies and analytical tools for pathogen detection in urban water systems: Progress and future perspectives

Y Zhao, F Huang, W Wang, R Gao, L Fan… - Science of the Total …, 2023 - Elsevier
The ubiquitous presence of pathogenic microorganisms, such as viruses, bacteria, fungi,
and protozoa, in urban water systems poses a significant risk to public health. The …

Towards a unified system for detecting waterborne pathogens

TM Straub, DP Chandler - Journal of Microbiological Methods, 2003 - Elsevier
Currently, there is no single method to collect, process, and analyze a water sample for all
pathogenic microorganisms of interest. Some of the difficulties in developing a universal …

Detection of pathogens in water: from phylochips to qPCR to pyrosequencing

TG Aw, JB Rose - Current opinion in biotechnology, 2012 - Elsevier
Waterborne pathogens pose a significant threat to human health and a proper assessment
of microbial water quality is important for decision making regarding water infrastructure and …

Next-generation sequencing (NGS) in the microbiological world: How to make the most of your money

AT Vincent, N Derome, B Boyle, AI Culley… - Journal of …, 2017 - Elsevier
The Sanger sequencing method produces relatively long DNA sequences of unmatched
quality and has been considered for long time as the gold standard for sequencing DNA …

Molecular biology and DNA microarray technology for microbial quality monitoring of water

K Lemarchand, L Masson… - Critical reviews in …, 2004 - Taylor & Francis
Public concern over polluted water is a major environmental issue worldwide. Microbial
contamination of water arguably represents the most significant risk to human health on a …

Identification of eukaryotic microorganisms with 18S rRNA next-generation sequencing in wastewater treatment plants, with a more targeted NGS approach required …

A Zahedi, TL Greay, A Paparini, KL Linge, CA Joll… - Water research, 2019 - Elsevier
While some microbial eukaryotes can improve effluent quality in wastewater treatment plants
(WWTPs), eukaryotic waterborne pathogens are a threat to public health. This study aimed …