[PDF][PDF] Rapid and scalable characterization of CRISPR technologies using an E. coli cell-free transcription-translation system
CRISPR-Cas systems offer versatile technologies for genome engineering, yet their
implementation has been outpaced by ongoing discoveries of new Cas nucleases and anti …
implementation has been outpaced by ongoing discoveries of new Cas nucleases and anti …
Systematic discovery of natural CRISPR-Cas12a inhibitors
Cas12a (Cpf1) is a CRISPR-associated nuclease with broad utility for synthetic genome
engineering, agricultural genomics, and biomedical applications. Although bacteria …
engineering, agricultural genomics, and biomedical applications. Although bacteria …
[PDF][PDF] Kinetic basis for DNA target specificity of CRISPR-Cas12a
Class 2 CRISPR-Cas nucleases are programmable genome editing tools with promising
applications in human health and disease. However, DNA cleavage at off-target sites that …
applications in human health and disease. However, DNA cleavage at off-target sites that …
[HTML][HTML] A catalogue of biochemically diverse CRISPR-Cas9 orthologs
G Gasiunas, JK Young, T Karvelis… - Nature …, 2020 - nature.com
Bacterial Cas9 nucleases from type II CRISPR-Cas antiviral defence systems have been
repurposed as genome editing tools. Although these proteins are found in many microbes …
repurposed as genome editing tools. Although these proteins are found in many microbes …
Genome-wide specificities of CRISPR-Cas Cpf1 nucleases in human cells
BP Kleinstiver, SQ Tsai, MS Prew, NT Nguyen… - Nature …, 2016 - nature.com
The activities and genome-wide specificities of CRISPR-Cas Cpf1 nucleases are not well
defined. We show that two Cpf1 nucleases from Acidaminococcus sp. BV3L6 and …
defined. We show that two Cpf1 nucleases from Acidaminococcus sp. BV3L6 and …
Target-dependent nickase activities of the CRISPR–Cas nucleases Cpf1 and Cas9
BXH Fu, JD Smith, RT Fuchs, M Mabuchi… - Nature …, 2019 - nature.com
Clustered regularly interspaced short palindromic repeats (CRISPR) machineries are
prokaryotic immune systems that have been adapted as versatile gene editing and …
prokaryotic immune systems that have been adapted as versatile gene editing and …
CRISPR tools to control gene expression in bacteria
A Vigouroux, D Bikard - Microbiology and Molecular Biology …, 2020 - Am Soc Microbiol
SUMMARY CRISPR-Cas systems have been engineered as powerful tools to control gene
expression in bacteria. The most common strategy relies on the use of Cas effectors …
expression in bacteria. The most common strategy relies on the use of Cas effectors …
Exploiting CRISPR/C as systems for biotechnology
TR Sampson, DS Weiss - Bioessays, 2014 - Wiley Online Library
The Cas9 endonuclease is the central component of the Type II CRISPR/Cas system, a
prokaryotic adaptive restriction system against invading nucleic acids, such as those …
prokaryotic adaptive restriction system against invading nucleic acids, such as those …
[PDF][PDF] The revolution continues: newly discovered systems expand the CRISPR-Cas toolkit
CRISPR-Cas systems defend prokaryotes against bacteriophages and mobile genetic
elements and serve as the basis for revolutionary tools for genetic engineering. Class 2 …
elements and serve as the basis for revolutionary tools for genetic engineering. Class 2 …
[HTML][HTML] CRISPR-Cas orthologues and variants: optimizing the repertoire, specificity and delivery of genome engineering tools
A Cebrian-Serrano, B Davies - Mammalian Genome, 2017 - Springer
Robust and cost-effective genome editing in a diverse array of cells and model organisms is
now possible thanks to the discovery of the RNA-guided endonucleases of the CRISPR-Cas …
now possible thanks to the discovery of the RNA-guided endonucleases of the CRISPR-Cas …