Fast forward genetics based on virus-induced gene silencing
DC Baulcombe - Current opinion in plant biology, 1999 - Elsevier
Gene expression in plants can be suppressed in a sequencespecific manner by infection
with virus vectors carrying fragments of host genes. Recent developments have revealed …
with virus vectors carrying fragments of host genes. Recent developments have revealed …
Virus-induced gene silencing, a post transcriptional gene silencing method
Virus-induced gene silencing (VIGS) is one of the reverse genetics tools for analysis of gene
function that uses viral vectors carrying a target gene fragment to produce dsRNA which …
function that uses viral vectors carrying a target gene fragment to produce dsRNA which …
Applications and advantages of virus‐induced gene silencing for gene function studies in plants
Virus‐induced gene silencing (VIGS) is a recently developed gene transcript suppression
technique for characterizing the function of plant genes. The approach involves cloning a …
technique for characterizing the function of plant genes. The approach involves cloning a …
Efficient virus‐induced gene silencing in Arabidopsis using a 'one‐step'TYMV‐derived vector
S Pflieger, S Blanchet, L Camborde… - The Plant …, 2008 - Wiley Online Library
Virus‐induced gene silencing (VIGS) is an important tool for the analysis of gene function in
plants. This technique exploits recombinant viral vectors harbouring fragments of plant …
plants. This technique exploits recombinant viral vectors harbouring fragments of plant …
Viruses and gene silencing in plants
D Baulcombe - 100 Years of Virology: The Birth and Growth of a …, 1999 - Springer
Genetic engineering of virus resistance in plants may be conferred by transgenes based on
sequences from the viral genome. In many instances the underlying mechanism involves the …
sequences from the viral genome. In many instances the underlying mechanism involves the …
Virus‐mediated efficient induction of epigenetic modifications of endogenous genes with phenotypic changes in plants
A Kanazawa, J Inaba, H Shimura, S Otagaki… - The Plant …, 2011 - Wiley Online Library
Gene silencing through transcriptional repression can be induced by targeting double‐
stranded RNA (dsRNA) to a gene promoter. It has been reported that a transgene was …
stranded RNA (dsRNA) to a gene promoter. It has been reported that a transgene was …
The silence of genes in transgenic plants
M Stam, JNM Mol, JM Kooter - Annals of botany, 1997 - Elsevier
In genetically modified plants, the introduced transgenes are sometimes not expressed.
They can be silenced. Transgenes can also cause the silencing of endogenous plant genes …
They can be silenced. Transgenes can also cause the silencing of endogenous plant genes …
Virus-induced gene silencing: a versatile tool for discovery of gene functions in plants
A Purkayastha, I Dasgupta - Plant Physiology and Biochemistry, 2009 - Elsevier
Virus-induced gene silencing (VIGS) is a technology that exploits an antiviral defense
mechanism in plants as a tool for plant reverse genetics. VIGS circumvents the need for …
mechanism in plants as a tool for plant reverse genetics. VIGS circumvents the need for …
Suppression of virus accumulation in transgenic plants exhibiting silencing of nuclear genes.
JJ English, E Mueller, DC Baulcombe - The Plant Cell, 1996 - academic.oup.com
Homology-dependent gene silencing contributes to variation between transgenic plants with
respect to transgene and/or endogenous gene expression levels. Recent studies have …
respect to transgene and/or endogenous gene expression levels. Recent studies have …
Multiple virus resistance at a high frequency using a single transgene construct
E Bucher, D Lohuis… - Journal of General …, 2006 - microbiologyresearch.org
RNA silencing is a natural antiviral defence in plants, which can be exploited in transgenic
plants for preprogramming virus recognition and ensuring enhanced resistance. By …
plants for preprogramming virus recognition and ensuring enhanced resistance. By …