Structural information can help engineer enzymes. Usually, specific amino acids in particular
regions are targeted for functional reconstruction to enhance the catalytic performance …

The limitations associated with natural enzyme catalysis have triggered the rise of the field of
protein engineering. Traditional rational design was based on the analysis of protein …

Site-directed mutagenesis is still a very efficient strategy to elaborate improved enzymes.
Recently, advances have been made in developing rational strategies aimed at reshaping …

Directed evolution has matured in academia and industry as a versatile algorithm to
redesign enzymes to match demands in biotechnological applications (as documented by …

Designing efficient enzymes is a formidable challenge at the forefront of modern
biocatalysis. Here, we review recent developments in the field and illustrate how the …

The quest for an enzyme with desired property is high for biocatalyic production of valuable
products in industrial biotechnology. Synthetic biology and metabolic engineering also …

Computational protein design relies on several approximations, including the use of fixed
backbones and rotamers, to reduce protein design to a computationally tractable problem …

The creation of artificial enzymes is a key objective of computational protein design.
Although de novo enzymes have been successfully designed, these exhibit low catalytic …

Enzymes generated by natural recruitment and protein engineering have greatly contribute
in various sets of applications. However, their insufficient stability is a bottleneck that limit the …

The challenging field of de novo enzyme design is beginning to produce exciting results.
The application of powerful computational methods to functional protein design has recently …