Background In‐house real‐time PCR (qPCR) is increasingly used to diagnose the so‐called
endemic mycoses as commercial assays are not widely available. Objectives To compare …

Lack of sensitive diagnostic tests impairs the understanding of the epidemiology of
histoplasmosis, a disease whose burden is estimated to be largely underrated. Broad-range …

Background A multicenter study was conducted. A panel containing DNA from Histoplasma
capsulatum, as well as negative and cross-reaction controls, was sent to five different …

Histoplasmosis is considered one of the most important endemic and systemic mycoses
worldwide. Until now few molecular techniques have been developed for its diagnosis. The …

Coccidioidomycosis is a systemic disease caused by the fungi Coccidioides immitis and C.
posadasii. It is a prevalent disease in arid regions with high temperatures and low …

Laboratory diagnosis of histoplasmosis is based on various methods, including microscopy,
culture, antigen, and DNA detection of Histoplasma capsulatum var. capsulatum or …

Histoplasmosis is a systemic fungal disease caused by the pathogen Histoplasma spp. that
results in significant morbidity and mortality in persons with HIV/AIDS and can also affect …

The performance of repetitive-sequence-based PCR (rep-PCR) using the DiversiLab system
for identification of Coccidioides species, Blastomyces dermatitidis, and Histoplasma …

Background This study aimed at improving a real-time polymerase-chain-reaction (qPCR)
assay for the detection of Histoplasma capsulatum, a fungal pathogen that can cause severe …

PCR assays have not reached the same level of acceptance for the detection of human
fungal pathogens as for other micro-organisms, mainly because the low number of micro …