DNA–protein interactions are central to fundamental cellular processes, yet widely
implemented technologies for measuring these interactions on a genome scale in bacteria …

Protein–DNA interactions are central to many basic biological processes, including
transcription regulation, DNA replication, and DNA repair. Chromatin Immunoprecipitation …

Advances in sequencing technology have provided an unprecedented view of bacterial
diversity, along with a daunting number of novel genes. Within this new reality lies the …

Studies of genome regulation routinely use high-throughput DNA sequencing approaches to
determine where specific proteins interact with DNA, and they rely on DNA amplification and …

This units presents methods through which one may isolate and identify novel bacterial DNA‐
binding proteins. Briefly, the DNA sequence of interest is affixed to beads, and then …

Accurate identification of the DNA-binding sites of transcription factors and other DNA-
binding proteins on the genome is crucial to understanding their molecular interactions with …

Highlights•Transcriptional profiling using RNA-seq.•Dual RNA-seq provides novel insights
into host–pathogen interactions.•Uncovering regulatory networks with next-generation …

Bacterial pathogens subvert host cells by manipulating cellular pathways for survival and
replication; in turn, host cells respond to the invading pathogen through cascading changes …

Crosstalk between neighboring cells underlies many biological processes, including cell
signaling, proliferation and differentiation. Current single-cell genomic technologies profile …

The ability to simultaneously characterize the bacterial and host expression programs during
infection would facilitate a comprehensive understanding of pathogen–host interactions …