Reproducibility in research can be compromised by both biological and technical variation,
but most of the focus is on removing the latter. Here we investigate the effects of biological …

Highlights•Variation exists within genetically identical populations raised in nominally
identical environments.•This variation, called 'phenotypic variability,'is relevant in medicine …

Single-cell RNA sequencing (scRNA-seq) distinguishes cell types, states and lineages
within the context of heterogeneous tissues. However, current single-cell data cannot …

Science relies heavily on replicate measurements. Additional replicates generally yield
more accurate and reliable summary statistics in experimental work. But the straightforward …

Protein and messenger RNA (mRNA) copy numbers vary from cell to cell in isogenic
bacterial populations. However, these molecules often exist in low copy numbers and are …

Evolution of bacterial pathogen populations has been detected in a variety of ways including
phenotypic tests, such as metabolic activity, reaction to antisera and drug resistance and …

Common recommendations for cell line authentication, annotation and quality control fall
short addressing genetic heterogeneity. Within the Human Toxome Project, we demonstrate …

Somatic structural variants (SVs) are widespread in cancer, but their impact on disease
evolution is understudied due to a lack of methods to directly characterize their functional …

Therapeutic glycoproteins have played a major role in the commercial success of
biotechnology in the post-genomic era. But isolating recombinant mammalian cell lines for …

Current computational workflows for comparative analyses of single-cell datasets typically
use discrete clusters as input when testing for differential abundance among experimental …