A survey of tools for variant analysis of next-generation genome sequencing data

S Pabinger, A Dander, M Fischer… - Briefings in …, 2014 - academic.oup.com
Recent advances in genome sequencing technologies provide unprecedented opportunities
to characterize individual genomic landscapes and identify mutations relevant for diagnosis …

[HTML][HTML] An analytical framework for optimizing variant discovery from personal genomes

G Highnam, JJ Wang, D Kusler, J Zook… - Nature …, 2015 - nature.com
The standardization and performance testing of analysis tools is a prerequisite to
widespread adoption of genome-wide sequencing, particularly in the clinic. However …

[HTML][HTML] SeqMule: automated pipeline for analysis of human exome/genome sequencing data

Y Guo, X Ding, Y Shen, GJ Lyon, K Wang - Scientific reports, 2015 - nature.com
Next-generation sequencing (NGS) technology has greatly helped us identify disease-
contributory variants for Mendelian diseases. However, users are often faced with issues …

An efficient and scalable analysis framework for variant extraction and refinement from population-scale DNA sequence data

G Jun, MK Wing, GR Abecasis, HM Kang - Genome research, 2015 - genome.cshlp.org
The analysis of next-generation sequencing data is computationally and statistically
challenging because of the massive volume of data and imperfect data quality. We present …

Using VarScan 2 for germline variant calling and somatic mutation detection

DC Koboldt, DE Larson… - Current protocols in …, 2013 - Wiley Online Library
The identification of small sequence variants remains a challenging but critical step in the
analysis of next‐generation sequencing data. Our variant‐calling tool, VarScan 2, employs …

[HTML][HTML] Detection of genomic structural variants from next-generation sequencing data

L Tattini, R D'Aurizio, A Magi - Frontiers in bioengineering and …, 2015 - frontiersin.org
Structural variants are genomic rearrangements larger than 50 bp accounting for around 1%
of the variation among human genomes. They impact on phenotypic diversity and play a role …

[HTML][HTML] An integrative variant analysis suite for whole exome next-generation sequencing data

D Challis, J Yu, US Evani, AR Jackson, S Paithankar… - BMC …, 2012 - Springer
Background Whole exome capture sequencing allows researchers to cost-effectively
sequence the coding regions of the genome. Although the exome capture sequencing …

A practical guide to filtering and prioritizing genetic variants

MJ Sefid Dashti, J Gamieldien - Biotechniques, 2017 - Taylor & Francis
Next-generation sequencing (NGS) of whole genomes and exomes is a powerful tool in
biomedical research and clinical diagnostics. However, the vast amount of data produced by …

[HTML][HTML] Sarek: A portable workflow for whole-genome sequencing analysis of germline and somatic variants

M Garcia, S Juhos, M Larsson, PI Olason… - …, 2020 - ncbi.nlm.nih.gov
Whole-genome sequencing (WGS) is a fundamental technology for research to advance
precision medicine, but the limited availability of portable and user-friendly workflows for …

[HTML][HTML] Identifying disease mutations in genomic medicine settings: current challenges and how to accelerate progress

GJ Lyon, K Wang - Genome medicine, 2012 - Springer
The pace of exome and genome sequencing is accelerating, with the identification of many
new disease-causing mutations in research settings, and it is likely that whole exome or …