Fluorescence microscopy is an invaluable tool in the biosciences, a genuine workhorse
technique offering exceptional contrast in conjunction with high specificity of labelling with …

Fluorescence, the absorption and re-emission of photons with longer wavelengths, is one of
those amazing phenomena of Nature. Its discovery and utilization had, and still has, a major …

Single-molecule fluorescence microscopy is currently one of the most popular methods in
the single-molecule toolbox. In this review, we discuss recent advances in fluorescence …

Super-resolution fluorescence microscopy is a powerful tool to visualize biomolecules and
cellular structures at the nanometer scale. Employing these techniques in living cells has …

Decoding cellular processes requires visualization of the spatial distribution and dynamic
interactions of biomolecules. It is therefore not surprising that innovations in imaging …

Fluorescence microscopy has undergone a renaissance in the last decade. The introduction
of green fluorescent protein (GFP) and two-photon microscopy has allowed systematic …

For centuries, cell biology has been based on light microscopy and at the same time been
limited by its optical resolution. However, several new technologies have been developed …

The light microscope has long been used to document the localization of fluorescent
molecules in cell biology research. With advances in digital cameras and the discovery and …

Δxmin, Δymin ¼ λ/2n sin α ð Þ λ/2 where λ is the wavelength of the light, n the index of
refraction of the microscopy medium and α half the aperture angle of the objective lens.[1] …

Fluorescence light microscopy is an indispensable approach for the investigation of cell
biological mechanisms. With the development of cutting-edge tools such as genetically …