RNA-guided programmable nucleases from CRISPR systems generate precise breaks in
DNA or RNA at specified positions. In cells, this activity can lead to changes in DNA …

Base editing is a genome editing strategy that induces specific single-nucleotide changes
within genomic DNA. Two major DNA base editors, cytosine base editors and adenine base …

RNA-guided nucleases of the CRISPR/Cas type can be repurposed as programmable
nucleotide deaminases to mediate targeted nucleotide substitutions. Such base editors have …

The past several years have seen an explosion in development of applications for the
CRISPR-Cas9 system, from efficient genome editing, to high-throughput screening, to …

DNA-editing enzymes perform chemical reactions on DNA nucleobases. These reactions
can change the genetic identity of the modified base or modulate gene expression. Interest …

Base editing—the introduction of single-nucleotide variants (SNVs) into DNA or RNA in
living cells—is one of the most recent advances in the field of genome editing. As around …

Genome editing methods have commonly relied on the initial introduction of double-
stranded DNA breaks (DSBs), resulting in stochastic insertions, deletions, and translocations …

Genome editing with CRISPR/Cas has rapidly gained popularity. Base editing, a new
CRISPR/Cas-based approach, can precisely convert one nucleotide to another in DNA or …

Genome editing has transformed the life sciences and has exciting prospects for use in
treating genetic diseases. Our laboratory developed base editing to enable precise and …

Existing adenine and cytosine base editors induce only a single type of modification, limiting
the range of DNA alterations that can be created. Here we describe a CRISPR–Cas9-based …