Traditional methods for cell cycle stage classification rely heavily on fluorescence
microscopy to monitor nuclear dynamics. These methods inevitably face the typical …

Cell cycle kinetics are crucial to cell fate decisions. Although live imaging has provided
extensive insights into this relationship at the single-cell level, the limited number of …

Due to its specificity, fluorescence microscopy has become a quintessential imaging tool in
cell biology. However, photobleaching, phototoxicity, and related artifacts continue to limit …

In the past decades, there has been an amazing progress in the understanding of the
molecular mechanisms of the cell cycle. This has been possible largely due to a better …

To understand complex biological processes, scientists must gain insight into the function of
individual living cells. In contrast to the imaging of fixed cells, where a single snapshot of the …

Existing approaches to evaluate cell viability involve cell staining with chemical reagents.
However, the step of exogenous staining makes these methods undesirable for rapid …

The cell-cycle transition from G 1 to S phase has been difficult to visualize. We have
harnessed antiphase oscillating proteins that mark cell-cycle transitions in order to develop …

Imaging flow cytometry combines the high-throughput capabilities of conventional flow
cytometry with single-cell imaging. Here we demonstrate label-free prediction of DNA …

Label-free imaging uses inherent contrast mechanisms within cells to create image contrast
without introducing dyes/labels, which may confound results. Quantitative phase imaging is …

Quantitative measurement of cell cycle progression in individual cells over time is important
in understanding drug treatment effects on cancer cells. Recent advances in time-lapse …