Improving the reliability of eDNA data interpretation

A Burian, Q Mauvisseau, M Bulling… - Molecular Ecology …, 2021 - Wiley Online Library
Global declines in biodiversity highlight the need to effectively monitor the density and
distribution of threatened species. In recent years, molecular survey methods detecting DNA …

Validating metabarcoding-based biodiversity assessments with multi-species occupancy models: a case study using coastal marine eDNA

B McClenaghan, ZG Compson, M Hajibabaei - PloS one, 2020 - journals.plos.org
Environmental DNA (eDNA) metabarcoding is an increasingly popular method for rapid
biodiversity assessment. As with any ecological survey, false negatives can arise during …

How to limit false positives in environmental DNA and metabarcoding?

GF Ficetola, P Taberlet, E Coissac - 2016 - Wiley Online Library
Environmental DNA (eDNA) and metabarcoding are boosting our ability to acquire data on
species distribution in a variety of ecosystems. Nevertheless, as most of sampling …

Replication levels, false presences and the estimation of the presence/absence from eDNA metabarcoding data

GF Ficetola, J Pansu, A Bonin… - Molecular ecology …, 2015 - Wiley Online Library
Environmental DNA (eDNA) metabarcoding is increasingly used to study the present and
past biodiversity. eDNA analyses often rely on amplification of very small quantities or …

A systematic review of sources of variability and uncertainty in eDNA data for environmental monitoring

C Mathieu, SM Hermans, G Lear, TR Buckley… - Frontiers in Ecology …, 2020 - frontiersin.org
Environmental DNA (eDNA) is becoming a standard tool in environmental monitoring that
aims to quantify spatiotemporal variation for the measurement and prediction of ecosystem …

Optimising sampling and analysis protocols in environmental DNA studies

A Buxton, E Matechou, J Griffin, A Diana… - Scientific Reports, 2021 - nature.com
Ecological surveys risk incurring false negative and false positive detections of the target
species. With indirect survey methods, such as environmental DNA, such error can occur at …

Uses and misuses of environmental DNA in biodiversity science and conservation

ME Cristescu, PDN Hebert - Annual Review of Ecology …, 2018 - annualreviews.org
The study of environmental DNA (eDNA) has the potential to revolutionize biodiversity
science and conservation action by enabling the census of species on a global scale in near …

Seasonality, DNA degradation and spatial heterogeneity as drivers of eDNA detection dynamics

CR Troth, MJ Sweet, J Nightingale, A Burian - Science of the Total …, 2021 - Elsevier
In recent years, eDNA-based assessments have evolved as valuable tools for research and
conservation. Most eDNA-based applications rely on comparisons across time or space …

Sampling designs for landscape‐level eDNA monitoring programs

RA Erickson, CM Merkes… - Integrated Environmental …, 2019 - Wiley Online Library
Effective natural resources management requires accurate information about species
distributions. Environmental DNA (eDNA) analysis is a commonly used method to determine …

Extracting abundance information from DNA‐based data

M Luo, Y Ji, D Warton, DW Yu - Molecular Ecology Resources, 2023 - Wiley Online Library
The accurate extraction of species‐abundance information from DNA‐based data
(metabarcoding, metagenomics) could contribute usefully to diet analysis and food‐web …