Much of the complex process of RNP biogenesis takes place at the gene cotranscriptionally.
The target for RNA binding and processing factors is, therefore, not a solitary RNA molecule …

The regulation of transcription elongation within the context of chromatin is a topic of great
interest. Even though chromatin presents a barrier to transcription by the PolII machinery in …

The synthesis of a messenger RNA in the nucleus of a eukaryotic cell is an immensely
complex undertaking. Each step in the pathway requires an enormous number of protein …

As the relationship between nuclear structure and function begins to unfold, a picture is
emerging of a dynamic landscape that is centred on the two main processes that execute the …

Abstract RNA polymerase II (RNAPII) passes through the nucleosome in a coordinated
manner, generating several intermediate nucleosomal states as it breaks and then reforms …

The landmark 1969 discovery of nuclear RNA polymerases I, II and III in diverse eukaryotes
represented a major turning point in the field that, with subsequent elucidation of the distinct …

Transcription is a fundamental step in gene expression, yet it remains poorly understood at a
cellular level. Visualization of transcription sites and active genes has led to the suggestion …

Nascent transcription occurs at nuclear foci of concentrated, hyperphosphorylated RNA
polymerase II (RNAPII). We investigate RNAPII localization, distal gene coassociation, and …

Regulation of eukaryotic gene expression is far more complex than one might have
imagined 30 years ago. However, progress towards understanding gene regulatory …

Transcriptional regulation in eukaryotes involves structurally and functionally distinct nuclear
RNA polymerases, corresponding general initiation factors, gene-specific (DNA-binding) …