Potential and limitations of microscopy and Raman spectroscopy for live-cell analysis of 3D cell cultures

V Charwat, K Schütze, W Holnthoner… - Journal of …, 2015 - Elsevier
Today highly complex 3D cell culture formats that closely mimic the in vivo situation are
increasingly available. Despite their wide use, the development of analytical methods and …

Devices and techniques used to obtain and analyze three‐dimensional cell cultures

G Agrawal, A Ramesh, P Aishwarya… - Biotechnology …, 2021 - Wiley Online Library
Cell cultures are indispensable for both basic and applied research. Advancements in cell
culture and analysis increase their utility for basic research and translational applications. A …

Single‐cell Raman microscopy of microengineered cell scaffolds

SJ Baldock, ACS Talari, R Smith… - Journal of Raman …, 2019 - Wiley Online Library
Studying cells in a three‐dimensional (3D) environment has great potential in understanding
cell behaviours such as morphology, proliferation, differentiation, and migration …

Advances in single cell Raman spectroscopy technologies for biological and environmental applications

D Wang, P He, Z Wang, G Li, N Majed, AZ Gu - Current opinion in …, 2020 - Elsevier
The increasing sophistication of single cell Raman spectroscopy (SCRS) via its integrations
with other advanced analytical techniques and modern data analytics, enable …

In situ characterisation of living cells by Raman spectroscopy

I Notingher, S Verrier, H Romanska, AE Bishop… - …, 2002 - content.iospress.com
We report the first Raman spectra of individual living and dead cells (MLE‐12 line) cultured
on bioinert standard poly‐L‐lysine coated fused silica and on bioactive 45S5 Bioglass® …

Recent developments in Raman spectral analysis of microbial single cells: Techniques and applications

H Jayan, H Pu, DW Sun - Critical Reviews in Food Science and …, 2022 - Taylor & Francis
The conventional microbial cell analyses are mostly population-averaged methods that
conceal the characteristics of single-cell in the community. Single-cell analysis can provide …

Monitoring of heat-and light exposure of cell culture media by RAMAN spectroscopy: Towards an analytical tool for cell culture media quality control

P Mayrhofer, D Reinhart, A Castan, R Kunert - Biochemical Engineering …, 2021 - Elsevier
Cell culture media are highly complex solutions of multiple nutrients for mammalian cells
and lay the foundation for enhanced quality of the expressed protein and the performance of …

Raman spectroscopy detects biochemical changes due to different cell culture environments in live cells in vitro

M Gargotti, E Efeoglu, HJ Byrne, A Casey - Analytical and bioanalytical …, 2018 - Springer
The in vitro cell culture environment can impact on cell biochemistry and cell cycle. The
manifestation of such substrate-induced changes in cell cycle in the Raman …

[HTML][HTML] High resolution Raman spectroscopy mapping of stem cell micropatterns

TC von Erlach, MAB Hedegaard, MM Stevens - Analyst, 2015 - pubs.rsc.org
We report on the use of high resolution Raman spectroscopy mapping combined with a
micro-engineered stem cell platform. This technique obtains quantitative information about …

Methods and applications of Raman microspectroscopy to single-cell analysis

IW Schie, T Huser - Applied spectroscopy, 2013 - journals.sagepub.com
Raman spectroscopy is a powerful biochemical analysis technique that allows for the
dynamic characterization and imaging of living biological cells in the absence of fluorescent …