[HTML][HTML] Luminescence lifetime encoding in time-domain flow cytometry
D Kage, K Hoffmann, M Wittkamp, J Ameskamp… - Scientific reports, 2018 - nature.com
Time-resolved flow cytometry represents an alternative to commonly applied spectral or
intensity multiplexing in bioanalytics. At present, the vast majority of the reports on this topic …
intensity multiplexing in bioanalytics. At present, the vast majority of the reports on this topic …
[HTML][HTML] Tempo-spectral multiplexing in flow cytometry with lifetime detection using QD-encoded polymer beads
D Kage, K Hoffmann, G Nifontova, V Krivenkov… - Scientific Reports, 2020 - nature.com
Semiconductor quantum dots (QDs) embedded into polymer microbeads are known to be
very attractive emitters for spectral multiplexing and colour encoding. Their luminescence …
very attractive emitters for spectral multiplexing and colour encoding. Their luminescence …
Expanding the potential of standard flow cytometry by extracting fluorescence lifetimes from cytometric pulse shifts
R Cao, MA Naivar, M Wilder, JP Houston - Cytometry Part A, 2014 - Wiley Online Library
Fluorescence lifetime measurements provide information about the fluorescence relaxation,
or intensity decay, of organic fluorophores, fluorescent proteins, and other inorganic …
or intensity decay, of organic fluorophores, fluorescent proteins, and other inorganic …
Capture of fluorescence decay times by flow cytometry
JP Houston, MA Naivar, P Jenkins… - Current protocols in …, 2012 - Wiley Online Library
In flow cytometry, the fluorescence decay time of an excitable species has been largely
underutilized and is not likely found as a standard parameter on any imaging cytometer …
underutilized and is not likely found as a standard parameter on any imaging cytometer …
Time-resolved microfluidic flow cytometer for decoding luminescence lifetimes in the microsecond region
Time-resolved luminescence detection using long-lived probes with lifetimes in the
microsecond region have shown great potential in ultrasensitive and multiplexed …
microsecond region have shown great potential in ultrasensitive and multiplexed …
[HTML][HTML] Lifetime encoding in flow cytometry for bead-based sensing of biomolecular interaction
D Kage, K Hoffmann, H Borcherding, U Schedler… - Scientific reports, 2020 - nature.com
To demonstrate the potential of time-resolved flow cytometry (FCM) for bioanalysis, clinical
diagnostics, and optically encoded bead-based assays, we performed a proof-of-principle …
diagnostics, and optically encoded bead-based assays, we performed a proof-of-principle …
Fluorescence Lifetime Measurements and Analyses: Protocols Using Flow Cytometry and High-Throughput Microscopy
JP Houston, S Valentino, A Bitton - Flow Cytometry Protocols, 2024 - Springer
This chapter focuses on applications and protocols that involve the measurement of the
fluorescence lifetime as an informative cytometric parameter. The timing of fluorescence …
fluorescence lifetime as an informative cytometric parameter. The timing of fluorescence …
Toward the measurement of multiple fluorescence lifetimes in flow cytometry: maximizing multi‐harmonic content from cells and microspheres
P Jenkins, MA Naivar, JP Houston - Journal of biophotonics, 2015 - Wiley Online Library
Flow cytometry is a powerful means for in vitro cellular analyses where multi‐fluorescence
and multi‐angle light scattering can indicate unique biochemical or morphological features …
and multi‐angle light scattering can indicate unique biochemical or morphological features …
Overview of fluorescence lifetime measurements in flow cytometry
The focus of this chapter is time-resolved flow cytometry, which is broadly defined as the
ability to measure the timing of fluorescence decay from excited fluorophores that pass …
ability to measure the timing of fluorescence decay from excited fluorophores that pass …
Flow cytometric time-resolved measurements by frequency heterodyning of fluorescence emission signals
JA Steinkamp, JD Parson - Optical Diagnostics of Living Cells …, 2001 - spiedigitallibrary.org
A first generation phase-sensitive flow cytometer has been developed that combines flow
cytometry (FCM) and fluorescence lifetime spectroscopy measurement principles to provide …
cytometry (FCM) and fluorescence lifetime spectroscopy measurement principles to provide …