Chinese hamster ovary cells (CHO) have been extensively utilized as the production
platform for therapeutic proteins including monoclonal antibodies in pharmaceutical …

A high‐cell‐density transient transfection system was recently developed in our laboratory
based on a CHO‐GS‐KO cell line. This method yields monoclonal antibody titers up to 350 …

During early preclinical development of therapeutic proteins, representative materials are
often required for process development, such as for pharmacokinetic/pharmacodynamic …

Transient gene expression (TGE) is a rapid method for the production of recombinant
proteins in mammalian cells. While the volumetric productivity of TGE has improved …

Abstract Chinese Hamster Ovary (CHO) cells are the principal mammalian host used for
stable cell line generation and biotherapeutic protein production. Until recently, production …

Transient expression of heterologous proteins in mammalian systems is a powerful way to
generate protein reagents quickly. However, it has historically suffered from poor yields in …

To rapidly produce large amounts of recombinant proteins, the generation of stable Chinese
Hamster Ovary (CHO) cell pools represents a useful alternative to large-scale transient gene …

Here we describe a simplified method for transient gene expression (TGE) in suspension-
adapted Chinese hamster ovary (CHO) cells using polyethylenimine (PEI) for DNA delivery …

An efficient rapid protein expression system is crucial to support early drug development.
Transient gene expression is an effective route, and to facilitate the use of the same host …

Fed-batch culture is the most commonly used upstream process in industry today for
recombinant monoclonal antibody production using Chinese hamster ovary (CHO) cells …