[HTML][HTML] 100 Hz ROCS microscopy correlated with fluorescence reveals cellular dynamics on different spatiotemporal scales

F Jünger, D Ruh, D Strobel, R Michiels, D Huber… - Nature …, 2022 - nature.com
Fluorescence techniques dominate the field of live-cell microscopy, but bleaching and
motion blur from too long integration times limit dynamic investigations of small objects. High …

Superior contrast and resolution by image formation in rotating coherent scattering (ROCS) microscopy

D Ruh, J Mutschler, M Michelbach, A Rohrbach - Optica, 2018 - opg.optica.org
Small structures, as inside living cells, move on millisecond timescales, which is usually far
beyond the imaging rate of superresolution fluorescence microscopes. In contrast, label-free …

Strong cytoskeleton activity on millisecond timescales upon particle binding revealed by ROCS microscopy

F Jünger, A Rohrbach - Cytoskeleton, 2018 - Wiley Online Library
Cells change their shape within seconds, cellular protrusions even on subsecond
timescales enabling various responses to stimuli of approaching bacteria, viruses or …

[HTML][HTML] Fast, label-free super-resolution live-cell imaging using rotating coherent scattering (ROCS) microscopy

F Jünger, P Olshausen, A Rohrbach - Scientific reports, 2016 - nature.com
Living cells are highly dynamic systems with cellular structures being often below the optical
resolution limit. Super-resolution microscopes, usually based on fluorescence cell labelling …

[HTML][HTML] Label-free imaging and bending analysis of microtubules by ROCS microscopy and optical trapping

MD Koch, A Rohrbach - Biophysical journal, 2018 - cell.com
Mechanical manipulation of single cytoskeleton filaments and their monitoring over long
times is difficult because of fluorescence bleaching or phototoxic protein degradation. The …

Four-dimensional imaging and quantitative reconstruction to analyse complex spatiotemporal processes in live cells

D Gerlich, J Beaudouin, M Gebhard, J Ellenberg… - Nature cell …, 2001 - nature.com
Live-cell imaging technology using fluorescent proteins (green fluorescent protein and its
homologues) has revolutionized the study of cellular dynamics,. But tools that can …

[HTML][HTML] Visualizing intracellular organelle and cytoskeletal interactions at nanoscale resolution on millisecond timescales

Y Guo, D Li, S Zhang, Y Yang, JJ Liu, X Wang, C Liu… - Cell, 2018 - cell.com
In eukaryotic cells, organelles and the cytoskeleton undergo highly dynamic yet organized
interactions capable of orchestrating complex cellular functions. Visualizing these …

Live cell imaging with chemical specificity using dual frequency CARS microscopy

I Pope, W Langbein, P Borri, P Watson - Methods in Enzymology, 2012 - Elsevier
Live cell microscopy using fluorescent proteins and small fluorescent probes is a well-
established and essential tool for cell biology; however, there is a considerable need for …

High‐speed broadband Fourier‐transform coherent anti‐stokes Raman scattering spectral microscopy

R Kinegawa, K Hiramatsu, K Hashimoto… - Journal of Raman …, 2019 - Wiley Online Library
We demonstrate broadband Fourier‐transform coherent anti‐Stokes Raman scattering (FT‐
CARS) spectral microscopy with a pixel dwell time of 42 μs, which is~ 50 times shorter than …

[HTML][HTML] Recent advances in imaging subcellular processes

KA Myers, C Janetopoulos - F1000Research, 2016 - ncbi.nlm.nih.gov
Cell biology came about with the ability to first visualize cells. As microscopy techniques
advanced, the early microscopists became the first cell biologists to observe the inner …