[HTML][HTML] 100 Hz ROCS microscopy correlated with fluorescence reveals cellular dynamics on different spatiotemporal scales
F Jünger, D Ruh, D Strobel, R Michiels, D Huber… - Nature …, 2022 - nature.com
Fluorescence techniques dominate the field of live-cell microscopy, but bleaching and
motion blur from too long integration times limit dynamic investigations of small objects. High …
motion blur from too long integration times limit dynamic investigations of small objects. High …
Superior contrast and resolution by image formation in rotating coherent scattering (ROCS) microscopy
D Ruh, J Mutschler, M Michelbach, A Rohrbach - Optica, 2018 - opg.optica.org
Small structures, as inside living cells, move on millisecond timescales, which is usually far
beyond the imaging rate of superresolution fluorescence microscopes. In contrast, label-free …
beyond the imaging rate of superresolution fluorescence microscopes. In contrast, label-free …
Strong cytoskeleton activity on millisecond timescales upon particle binding revealed by ROCS microscopy
F Jünger, A Rohrbach - Cytoskeleton, 2018 - Wiley Online Library
Cells change their shape within seconds, cellular protrusions even on subsecond
timescales enabling various responses to stimuli of approaching bacteria, viruses or …
timescales enabling various responses to stimuli of approaching bacteria, viruses or …
[HTML][HTML] Fast, label-free super-resolution live-cell imaging using rotating coherent scattering (ROCS) microscopy
F Jünger, P Olshausen, A Rohrbach - Scientific reports, 2016 - nature.com
Living cells are highly dynamic systems with cellular structures being often below the optical
resolution limit. Super-resolution microscopes, usually based on fluorescence cell labelling …
resolution limit. Super-resolution microscopes, usually based on fluorescence cell labelling …
[HTML][HTML] Label-free imaging and bending analysis of microtubules by ROCS microscopy and optical trapping
MD Koch, A Rohrbach - Biophysical journal, 2018 - cell.com
Mechanical manipulation of single cytoskeleton filaments and their monitoring over long
times is difficult because of fluorescence bleaching or phototoxic protein degradation. The …
times is difficult because of fluorescence bleaching or phototoxic protein degradation. The …
Four-dimensional imaging and quantitative reconstruction to analyse complex spatiotemporal processes in live cells
D Gerlich, J Beaudouin, M Gebhard, J Ellenberg… - Nature cell …, 2001 - nature.com
Live-cell imaging technology using fluorescent proteins (green fluorescent protein and its
homologues) has revolutionized the study of cellular dynamics,. But tools that can …
homologues) has revolutionized the study of cellular dynamics,. But tools that can …
[HTML][HTML] Visualizing intracellular organelle and cytoskeletal interactions at nanoscale resolution on millisecond timescales
In eukaryotic cells, organelles and the cytoskeleton undergo highly dynamic yet organized
interactions capable of orchestrating complex cellular functions. Visualizing these …
interactions capable of orchestrating complex cellular functions. Visualizing these …
Live cell imaging with chemical specificity using dual frequency CARS microscopy
Live cell microscopy using fluorescent proteins and small fluorescent probes is a well-
established and essential tool for cell biology; however, there is a considerable need for …
established and essential tool for cell biology; however, there is a considerable need for …
High‐speed broadband Fourier‐transform coherent anti‐stokes Raman scattering spectral microscopy
We demonstrate broadband Fourier‐transform coherent anti‐Stokes Raman scattering (FT‐
CARS) spectral microscopy with a pixel dwell time of 42 μs, which is~ 50 times shorter than …
CARS) spectral microscopy with a pixel dwell time of 42 μs, which is~ 50 times shorter than …
[HTML][HTML] Recent advances in imaging subcellular processes
KA Myers, C Janetopoulos - F1000Research, 2016 - ncbi.nlm.nih.gov
Cell biology came about with the ability to first visualize cells. As microscopy techniques
advanced, the early microscopists became the first cell biologists to observe the inner …
advanced, the early microscopists became the first cell biologists to observe the inner …