Highly efficient gene knockout in mice and zebrafish with RNA-guided endonucleases
YH Sung, JM Kim, HT Kim, J Lee, J Jeon, Y Jin… - Genome …, 2014 - genome.cshlp.org
RNA-guided endonucleases (RGENs), derived from the prokaryotic Type II CRISPR-Cas
system, enable targeted genome modification in cells and organisms. Here we describe the …
system, enable targeted genome modification in cells and organisms. Here we describe the …
[HTML][HTML] Efficient in vivo genome editing using RNA-guided nucleases
Clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated
(Cas) systems have evolved in bacteria and archaea as a defense mechanism to silence …
(Cas) systems have evolved in bacteria and archaea as a defense mechanism to silence …
Heritable gene targeting in the mouse and rat using a CRISPR-Cas system
To the Editor: CRISPR-Cas systems have been developed as an efficient gene editing
technology in cells and model organisms. Here we use a CRISPR-Cas system to induce …
technology in cells and model organisms. Here we use a CRISPR-Cas system to induce …
Efficient genome editing in zebrafish using a CRISPR-Cas system
In bacteria, foreign nucleic acids are silenced by clustered, regularly interspaced, short
palindromic repeats (CRISPR)–CRISPR-associated (Cas) systems. Bacterial type II CRISPR …
palindromic repeats (CRISPR)–CRISPR-associated (Cas) systems. Bacterial type II CRISPR …
Maximizing mutagenesis with solubilized CRISPR-Cas9 ribonucleoprotein complexes
CRISPR-Cas9 enables efficient sequence-specific mutagenesis for creating somatic or
germline mutants of model organisms. Key constraints in vivo remain the expression and …
germline mutants of model organisms. Key constraints in vivo remain the expression and …
Analysis of off-target effects of CRISPR/Cas-derived RNA-guided endonucleases and nickases
RNA-guided endonucleases (RGENs), derived from the prokaryotic adaptive immune
system known as CRISPR/Cas, enable targeted genome engineering in cells and …
system known as CRISPR/Cas, enable targeted genome engineering in cells and …
Targeted genome engineering in human cells with the Cas9 RNA-guided endonuclease
We employ the CRISPR-Cas system of Streptococcus pyogenes as programmable RNA-
guided endonucleases (RGENs) to cleave DNA in a targeted manner for genome editing in …
guided endonucleases (RGENs) to cleave DNA in a targeted manner for genome editing in …
The new CRISPR–Cas system: RNA-guided genome engineering to efficiently produce any desired genetic alteration in animals
D Seruggia, L Montoliu - Transgenic research, 2014 - Springer
The CRISPR–Cas system is the newest targeted nuclease for genome engineering. In less
than 1 year, the ease, robustness and efficiency of this method have facilitated an immense …
than 1 year, the ease, robustness and efficiency of this method have facilitated an immense …
[PDF][PDF] Introducing a spectrum of long-range genomic deletions in human embryonic stem cells using type I CRISPR-Cas
CRISPR-Cas systems enable microbial adaptive immunity and provide eukaryotic genome
editing tools. These tools employ a single effector enzyme of type II or V CRISPR to generate …
editing tools. These tools employ a single effector enzyme of type II or V CRISPR to generate …
Genome-wide binding of the CRISPR endonuclease Cas9 in mammalian cells
Abstract Bacterial type II CRISPR-Cas9 systems have been widely adapted for RNA-guided
genome editing and transcription regulation in eukaryotic cells, yet their in vivo target …
genome editing and transcription regulation in eukaryotic cells, yet their in vivo target …