Minimizing inclusion body formation during recombinant protein production in Escherichia coli at bench and pilot plant scale
F Hoffmann, J van den Heuvel, N Zidek… - Enzyme and microbial …, 2004 - Elsevier
Many recombinant proteins partially aggregate into inclusion bodies during production in
Escherichia coli in batch culture on defined medium. Production on complex medium …
Escherichia coli in batch culture on defined medium. Production on complex medium …
Selective expression of the soluble product fraction in Escherichia coli cultures employed in recombinant protein production processes
S Gnoth, R Simutis, A Lübbert - Applied microbiology and biotechnology, 2010 - Springer
Recombinant proteins produced in Escherichia coli hosts may appear within the cells'
cytoplasm in form of insoluble inclusion bodies (IB's) and/or as dissolved functional protein …
cytoplasm in form of insoluble inclusion bodies (IB's) and/or as dissolved functional protein …
Use of a stress-minimisation paradigm in high cell density fed-batch Escherichia coli fermentations to optimise recombinant protein production
C Wyre, TW Overton - Journal of Industrial Microbiology and …, 2014 - academic.oup.com
Production of recombinant proteins is an industrially important technique in the
biopharmaceutical sector. Many recombinant proteins are problematic to generate in a …
biopharmaceutical sector. Many recombinant proteins are problematic to generate in a …
Bioprocessing of Therapeutic Proteins from the Inclusion Bodies of Escherichia coli
AK Panda - Biotechnology in India II, 2003 - Springer
Escherichia coli has been most extensively used for the large-scale production of
therapeutic proteins, which do not require complex glycosylation for bioactivity. In recent …
therapeutic proteins, which do not require complex glycosylation for bioactivity. In recent …
Effect of Modulated Glucose Uptake on High‐Level Recombinant Protein Production in a Dense Escherichia coli Culture
CH Chou, GN Bennett, KY San - Biotechnology progress, 1994 - Wiley Online Library
Methyl α‐glucoside (α‐MG) is a metabolically inert glucose analog sharing the same
phosphotransferase system with glucose. The potential of using this compound, which acts …
phosphotransferase system with glucose. The potential of using this compound, which acts …
The use of fed batch cultivation for achieving high cell densities in the production of a recombinant protein in Escherichia coli
L Strandberg, L Andersson… - FEMS microbiology …, 1994 - academic.oup.com
The production of the fusion protein staphylococcal protein A/E. coli β-galactosidase in
Escherichia coli was studied in batch and fed batch cultivations. Batch cultivation of a …
Escherichia coli was studied in batch and fed batch cultivations. Batch cultivation of a …
Effect of substrate feed rate on recombinant protein secretion, degradation and inclusion body formation in Escherichia coli
M Boström, K Markland, AM Sandén… - Applied microbiology …, 2005 - Springer
The effect of changes in substrate feed rate during fedbatch cultivation was investigated with
respect to soluble protein formation and transport of product to the periplasm in Escherichia …
respect to soluble protein formation and transport of product to the periplasm in Escherichia …
Boosting recombinant inclusion body production—from classical fed-batch approach to continuous cultivation
J Kopp, AM Kolkmann, PG Veleenturf… - … in Bioengineering and …, 2019 - frontiersin.org
State of the art microbial recombinant protein production is regularly performed in fed-batch
based cultivations. However, these cultivations suffer from highly time-dependent changes …
based cultivations. However, these cultivations suffer from highly time-dependent changes …
Inclusion body anatomy and functioning of chaperone-mediated in vivo inclusion body disassembly during high-level recombinant protein production in Escherichia …
U Rinas, F Hoffmann, E Betiku, D Estapé… - Journal of biotechnology, 2007 - Elsevier
During production in recombinant Escherichia coli, the human basic fibroblast growth factor
(hFGF-2) partly aggregates into stable cytoplasmic inclusion bodies. These inclusion bodies …
(hFGF-2) partly aggregates into stable cytoplasmic inclusion bodies. These inclusion bodies …
Enhancing Recombinant Protein Yields in the E. coli Periplasm by Combining Signal Peptide and Production Rate Screening
A Karyolaimos, H Ampah-Korsah, T Hillenaar… - Frontiers in …, 2019 - frontiersin.org
Proteins that contain disulfide bonds mainly mature in the oxidative environment of the
eukaryotic endoplasmic reticulum or the periplasm of Gram-negative bacteria. In E. coli …
eukaryotic endoplasmic reticulum or the periplasm of Gram-negative bacteria. In E. coli …
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