CRISPR/Cas9 based systems have emerged as versatile platforms for precision genome
editing in a wide range of organisms. Here we have developed powerful CRISPR/Cas9 tools …

Filamentous fungi play an important role in human health and industrial/agricultural
production. With the increasing number of full genomes available for fungal species, the …

The CRISPR/Cas9 system is a revolutionary genome editing tool. However, in eukaryotes,
search and optimization of a suitable promoter for guide RNA expression is a significant …

In recent years, a variety of genetic tools have been developed and applied to various
filamentous fungi, which are widely applied in agriculture and the food industry. However …

Filamentous fungi represent an invaluable source of pharmaceutically active compounds.
The development of versatile methods to genetically manipulate filamentous fungi is of great …

Filamentous fungi have wide applications in biotechnology. The CRISPR/Cas9 system is a
powerful genome-editing method that facilitates genetic alterations of genomes in a variety …

Background CRISPR/Cas9 mediated genome editing has expedited the way of constructing
multiple gene alterations in filamentous fungi, whereas traditional methods are time …

Due to their biotechnological relevance as well as their importance as disease agents,
filamentous fungi and oomycetes have been prime candidates for genetic selection and in …

DNA double‐strand break (DSB) repair induced by the RNA‐programmed nuclease Cas9
has become a popular method for genome editing. Direct genome editing via Cas9‐CRISPR …

Application of CRISPR-Cas9 systems has revolutionized genome editing across all domains
of life. Here we report implementation of the heterologous Type II CRISPR-Cas9 system in …