HEK293 cell line as a platform to produce recombinant proteins and viral vectors
E Tan, CSH Chin, ZFS Lim, SK Ng - Frontiers in bioengineering and …, 2021 - frontiersin.org
Animal cell-based expression platforms enable the production of complex biomolecules
such as recombinant proteins and viral vectors. Although most biotherapeutics are produced …
such as recombinant proteins and viral vectors. Although most biotherapeutics are produced …
Molecular characterization of HEK293 cells as emerging versatile cell factories
M Pulix, V Lukashchuk, DC Smith… - Current opinion in …, 2021 - Elsevier
Highlights•Manufacture of biopharmaceuticals, virus-like particles and viral vectors.•System
biology,'omics, cellular phenotype and cell engineering.•Cell line development, culture and …
biology,'omics, cellular phenotype and cell engineering.•Cell line development, culture and …
Affecting HEK293 cell growth and production performance by modifying the expression of specific genes
L Abaandou, D Quan, J Shiloach - Cells, 2021 - mdpi.com
The HEK293 cell line has earned its place as a producer of biotherapeutics. In addition to its
ease of growth in serum-free suspension culture and its amenability to transfection, this cell …
ease of growth in serum-free suspension culture and its amenability to transfection, this cell …
Human cell lines for biopharmaceutical manufacturing: history, status, and future perspectives
J Dumont, D Euwart, B Mei, S Estes… - Critical reviews in …, 2016 - Taylor & Francis
Biotherapeutic proteins represent a mainstay of treatment for a multitude of conditions, for
example, autoimmune disorders, hematologic disorders, hormonal dysregulation, cancers …
example, autoimmune disorders, hematologic disorders, hormonal dysregulation, cancers …
CHO cells in biotechnology for production of recombinant proteins: current state and further potential
Recombinant Chinese hamster ovary cells (rCHO) cells have been the most commonly used
mammalian host for large-scale commercial production of therapeutic proteins. Recent …
mammalian host for large-scale commercial production of therapeutic proteins. Recent …
Mammalian cell line developments in speed and efficiency
S Estes, M Melville - Mammalian cell cultures for biologics manufacturing, 2014 - Springer
<? xm-replace_text {Heading}?> Abstract Mammalian cell expression systems are the
dominant tool today for producing complex biotherapeutic proteins. In this chapter, we …
dominant tool today for producing complex biotherapeutic proteins. In this chapter, we …
Human cells: new platform for recombinant therapeutic protein production
K Swiech, V Picanço-Castro, DT Covas - Protein expression and …, 2012 - Elsevier
The demand for recombinant therapeutic proteins is significantly increasing. There is a
constant need to improve the existing expression systems, and also developing novel …
constant need to improve the existing expression systems, and also developing novel …
Advances in mammalian cell line development technologies for recombinant protein production
T Lai, Y Yang, SK Ng - Pharmaceuticals, 2013 - mdpi.com
From 2006 to 2011, an average of 15 novel recombinant protein therapeutics have been
approved by US Food and Drug Administration (FDA) annually. In addition, the expiration of …
approved by US Food and Drug Administration (FDA) annually. In addition, the expiration of …
25 years of recombinant proteins from reactor-grown cells—where do we go from here?
DL Hacker, M De Jesus, FM Wurm - Biotechnology advances, 2009 - Elsevier
The purpose of this review is to describe the current status and to highlight several emerging
trends in the manufacture of recombinant therapeutic proteins in cultivated mammalian cells …
trends in the manufacture of recombinant therapeutic proteins in cultivated mammalian cells …
Designing cell lines for viral vaccine production: Where do we stand?
Y Genzel - Biotechnology journal, 2015 - Wiley Online Library
Established animal cells, such as Vero, Madin Darby canine kidney (MDCK) or chicken
embryo fibroblasts (CEFs), are still the main cell lines used for viral vaccine production …
embryo fibroblasts (CEFs), are still the main cell lines used for viral vaccine production …