Expansion microscopy (ExM) allows scalable imaging of preserved 3D biological specimens
with nanoscale resolution on fast diffraction-limited microscopes. Here, we explore the utility …

The limited resolving power of conventional diffraction-limited microscopy hinders analysis
of small, densely packed structural elements in cells. Expansion microscopy (ExM) provides …

Highlights•Classical super-resolution methods are difficult to use across neural
circuits.•Expansion microscopy physically magnifies specimens to reveal nanoscale …

One of the biggest unsolved questions in neuroscience is how molecules and neuronal
circuitry create behaviors, and how their misregulation or dysfunction results in neurological …

Nanoscale resolution imaging of whole vertebrates is required for a systematic
understanding of human diseases, but this has yet to be realized. Expansion microscopy …

We recently developed expansion microscopy (ExM), which achieves nanoscale-precise
imaging of specimens at~ 70 nm resolution (with~ 4.5 x linear expansion) by isotropic …

Expansion microscopy (ExM) is a physical form of magnification that increases the effective
resolving power of any microscope. Here, we describe the fundamental principles of ExM, as …

Expansion microscopy (ExM) is a technique that physically expands preserved cells and
tissues before microscope imaging, so that conventional diffraction‐limited microscopes can …

Super-resolution microscopy reveals the molecular organization of biological structures
down to the nanoscale. While it allows the study of protein complexes in single cells, small …

Expansion microscopy (ExM) is a highly effective technique for super-resolution
fluorescence microscopy that enables imaging of biological samples beyond the diffraction …