A versatile reverse genetics platform for SARS-CoV-2 and other positive-strand RNA viruses
The current COVID-19 pandemic is caused by the severe acute respiratory syndrome
coronavirus 2 (SARS-CoV-2). We demonstrate that despite the large size of the viral RNA …
coronavirus 2 (SARS-CoV-2). We demonstrate that despite the large size of the viral RNA …
Rapid reconstruction of SARS-CoV-2 using a synthetic genomics platform
T Thi Nhu Thao, F Labroussaa, N Ebert, P V'kovski… - Nature, 2020 - nature.com
Reverse genetics has been an indispensable tool to gain insights into viral pathogenesis
and vaccine development. The genomes of large RNA viruses, such as those from …
and vaccine development. The genomes of large RNA viruses, such as those from …
Engineering SARS-CoV-2 using a reverse genetic system
Reverse genetic systems are a critical tool for studying viruses and identifying
countermeasures. In response to the ongoing COVID-19 pandemic, we recently developed …
countermeasures. In response to the ongoing COVID-19 pandemic, we recently developed …
Replication and single-cycle delivery of SARS-CoV-2 replicons
Molecular virology tools are critical for basic studies of the severe acute respiratory
syndrome coronavirus 2 (SARS-CoV-2) and for developing new therapeutics. Experimental …
syndrome coronavirus 2 (SARS-CoV-2) and for developing new therapeutics. Experimental …
[HTML][HTML] Establishment of a reverse genetics system for SARS-CoV-2 using circular polymerase extension reaction
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has been identified as the
causative agent of coronavirus disease 2019 (COVID-19). Although multiple mutations have …
causative agent of coronavirus disease 2019 (COVID-19). Although multiple mutations have …
An infectious cDNA clone of SARS-CoV-2
X Xie, A Muruato, KG Lokugamage, K Narayanan… - Cell host & …, 2020 - cell.com
The ongoing pandemic of COVID-19, caused by severe acute respiratory syndrome
coronavirus 2 (SARS-CoV-2), underscores the urgency to develop experimental systems for …
coronavirus 2 (SARS-CoV-2), underscores the urgency to develop experimental systems for …
Reverse-transcribed SARS-CoV-2 RNA can integrate into the genome of cultured human cells and can be expressed in patient-derived tissues
L Zhang, A Richards, MI Barrasa… - Proceedings of the …, 2021 - National Acad Sciences
Prolonged detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)
RNA and recurrence of PCR-positive tests have been widely reported in patients after …
RNA and recurrence of PCR-positive tests have been widely reported in patients after …
A plasmid DNA-launched SARS-CoV-2 reverse genetics system and coronavirus toolkit for COVID-19 research
The recent emergence of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-
2), the underlying cause of Coronavirus Disease 2019 (COVID-19), has led to a worldwide …
2), the underlying cause of Coronavirus Disease 2019 (COVID-19), has led to a worldwide …
Cloning of a passage-free SARS-CoV-2 genome and mutagenesis using red recombination
A Herrmann, D Jungnickl, A Cordsmeier… - International journal of …, 2021 - mdpi.com
The ongoing pandemic coronavirus (CoV) disease 2019 (COVID-19) by severe acute
respiratory syndrome CoV-2 (SARS-CoV-2) has already caused substantial morbidity …
respiratory syndrome CoV-2 (SARS-CoV-2) has already caused substantial morbidity …
Reverse genetic systems of SARS-CoV-2 for antiviral research
Reverse genetic systems are widely used to engineer recombinant viruses with desired
mutations. In response to the COVID-19 pandemic, four types of reverse genetic systems …
mutations. In response to the COVID-19 pandemic, four types of reverse genetic systems …