Site-specific conjugation of an antibody on a gold nanoparticle surface for one-step diagnosis of prostate specific antigen with dynamic light scattering

N Mustafaoglu, T Kiziltepe, B Bilgicer - Nanoscale, 2017 - pubs.rsc.org
Small dimensions of gold nanoparticles (AuNPs) necessitate antibodies to be immobilized in
an oriented fashion in order to conserve their antigen binding activity for proper function. In …

Chemical modification of antibodies enables the formation of stable antibody–gold nanoparticle conjugates for biosensing

SL Filbrun, AB Filbrun, FL Lovato, SH Oh, EA Driskell… - Analyst, 2017 - pubs.rsc.org
Antibody-modified gold nanoparticles (AuNPs) are central to many novel and emerging
biosensing technologies due to the specificity provided by antibody–antigen interactions …

A one-step homogeneous immunoassay for cancer biomarker detection using gold nanoparticle probes coupled with dynamic light scattering

X Liu, Q Dai, L Austin, J Coutts, G Knowles… - Journal of the …, 2008 - ACS Publications
A one-step homogeneous immunoassay for the detection of a prostate cancer biomarker,
free-PSA (prostate specific antigen), was developed using gold nanoparticle probes coupled …

Quantifying bound and active antibodies conjugated to gold nanoparticles: A comprehensive and robust approach to evaluate immobilization chemistry

K Tripathi, JD Driskell - ACS omega, 2018 - ACS Publications
Gold nanoparticles (AuNPs) functionalized with antibodies have the potential to improve
biosensing technology because of the unique optical properties of AuNPs and the specificity …

Controlled protein embedment onto Au/Ag core–shell nanoparticles for immuno-labeling of nanosilver surface

IH Lee, JM Lee, Y Jung - Acs Applied Materials & Interfaces, 2014 - ACS Publications
Difficulties in stable conjugation of biomolecules to nanosilver surfaces have severely
limited the use of silver nanostructures in biological applications. Here, we report a facile …

Gold nanoparticle-enabled blood test for early stage cancer detection and risk assessment

T Zheng, N Pierre-Pierre, X Yan, Q Huo… - … applied materials & …, 2015 - ACS Publications
When citrate ligands-capped gold nanoparticles are mixed with blood sera, a protein corona
is formed on the nanoparticle surface due to the adsorption of various proteins in the blood …

pH impacts the orientation of antibody adsorbed onto gold nanoparticles

G Ruiz, K Tripathi, S Okyem, JD Driskell - Bioconjugate chemistry, 2019 - ACS Publications
Novel detection strategies that exploit the unique properties of gold nanoparticles (AuNPs)
hold great promise for the advancement of diagnostic testing. Fundamental to many of these …

High-affinity points of interaction on antibody allow synthesis of stable and highly functional antibody–gold nanoparticle conjugates

S Okyem, O Awotunde, T Ogunlusi… - Bioconjugate …, 2021 - ACS Publications
Many emerging nanobiotechnologies rely on the proper function of proteins immobilized on
gold nanoparticles. Often, the surface chemistry of the AuNP is engineered to control the …

A new method for non-labeling attomolar detection of diseases based on an individual gold nanorod immunosensor

PL Truong, C Cao, S Park, M Kim, SJ Sim - Lab on a Chip, 2011 - pubs.rsc.org
Herein, we present the use of a single gold nanorod sensor for detection of diseases on an
antibody-functionalized surface, based on antibody–antigen interaction and the localized …

A fluorescence-based method to directly quantify antibodies immobilized on gold nanoparticles

SL Filbrun, JD Driskell - Analyst, 2016 - pubs.rsc.org
The ability to evaluate antibody immobilization onto gold nanoparticles is critical for
assessing coupling chemistry and optimizing the sensitivity of nanoparticle-enabled …