The critical roles of exposed surface residues for the thermostability and halotolerance of a novel GH11 xylanase from the metagenomic library of a saline-alkaline soil

Z Li, X Li, T Liu, S Chen, H Liu, H Wang, K Li… - International journal of …, 2019 - Elsevier
Thermostable and halotolerant GH11 xylanases are highly desirable in various industrial
applications but they were rarely characterized. In this study, we firstly revealed the genetic …

[HTML][HTML] Insights into the role of exposed surface charged residues in the alkali-tolerance of GH11 xylanase

X Wu, Q Zhang, L Zhang, S Liu, G Chen… - Frontiers in …, 2020 - frontiersin.org
Thermostable and alkaline-or acid-stable xylanases are more advantageous in agricultural
and industrial fields. In this study, a rational structure-based design was conducted based on …

[HTML][HTML] Engineering mesophilic GH11 xylanase from Cellulomonas flavigena by rational design of N-terminus substitution

W Tian, Z Zhang, C Yang, P Li, J Xiao… - … in Bioengineering and …, 2022 - frontiersin.org
Xylanase, a glycoside hydrolase, is widely used in the food, papermaking, and textile
industries; however, most xylanases are inactive at high temperatures. In this study, a …

[HTML][HTML] Clustered surface amino acid residues modulate the acid stability of GH10 xylanase in fungi

Y Xia, W Wang, Y Wei, C Guo, S Song, S Cai… - Applied Microbiology …, 2024 - Springer
Acidic xylanases are widely used in industries such as biofuels, animal feeding, and fruit
juice clarification due to their tolerance to acidic environments. However, the factors …

Enzymatic characterization of a novel thermostable and alkaline tolerant GH10 xylanase and activity improvement by multiple rational mutagenesis strategies

Z Lai, C Zhou, X Ma, Y Xue, Y Ma - International Journal of Biological …, 2021 - Elsevier
Thermo-alkaline xylanases are widely applied in paper pulping industry. In this study, a
novel thermostable and alkaline tolerant GH10 xylanase (Xyn30Y5) gene from alkaliphilic …

Characterization and structural analysis of a thermophilic GH11 xylanase from compost metatranscriptome

Y Yi, S Xu, A Kovalevsky, X Zhang, D Liu… - Applied Microbiology and …, 2021 - Springer
Xylanase is efficient for xylan degradation and widely applied in industries. We found a
GH11 family xylanase (Xyn11A) with high thermostability and catalytic activity from compost …

Enhancing thermal tolerance of a fungal GH11 xylanase guided by B-factor analysis and multiple sequence alignment

N Han, Y Ma, Y Mu, X Tang, J Li, Z Huang - Enzyme and Microbial …, 2019 - Elsevier
Xylanases, capable of hydrolyzing xylans which are abundant in nature, have been
employed as important biocatalyst in many industrial processes. Xylanases with …

Improvement of GH10 family xylanase thermostability by introducing of an extra α-helix at the C-terminal

G Li, X Chen, X Zhou, R Huang, L Li, Y Miao… - Biochemical and …, 2019 - Elsevier
Xylanase is an important enzyme in industrial applications, which usually require the
enzyme to maintain activity in high-temperature condition. In this study, a GH10 family …

Engineering a thermostable fungal GH10 xylanase, importance of N‐terminal amino acids

L Song, A Tsang, M Sylvestre - Biotechnology and …, 2015 - Wiley Online Library
Xylanases are used in many industrial processes including pulp bleaching, baking,
detergent, and the hydrolysis of plant cell wall in biofuels production. In this work we have …

[HTML][HTML] XynDZ5: a new thermostable GH10 xylanase

D Zarafeta, AP Galanopoulou, ME Leni… - Frontiers in …, 2020 - frontiersin.org
Xylanolytic enzymes have a broad range of applications in industrial biotechnology as
biocatalytic components of various processes and products, such as food additives, bakery …