Exploring the synthetic biology potential of bacteriophages for engineering non-model bacteria

EM Lammens, PI Nikel, R Lavigne - Nature communications, 2020 - nature.com
Non-model bacteria like Pseudomonas putida, Lactococcus lactis and other species have
unique and versatile metabolisms, offering unique opportunities for Synthetic Biology …

[HTML][HTML] Bacteriophage-based synthetic biology for the study of infectious diseases

RJ Citorik, M Mimee, TK Lu - Current opinion in microbiology, 2014 - Elsevier
Highlights•Multidrug-resistant infections have sparked a renewed interest in
bacteriophages.•Synthetic biology has enabled technologies for next-generation phage …

Phage therapy in the era of synthetic biology

EM Barbu, KC Cady, B Hubby - Cold Spring Harbor …, 2016 - cshperspectives.cshlp.org
For more than a century, bacteriophage (or phage) research has enabled some of the most
important discoveries in biological sciences and has equipped scientists with many of the …

Harnessing stepping-stone hosts to engineer, select, and reboot synthetic bacteriophages in one pot

L Cheng, Z Deng, H Tao, W Song, B Xing, W Liu… - Cell Reports …, 2022 - cell.com
Advances in synthetic genomics have led to a great demand for genetic manipulation.
Trimming any process to simplify and accelerate streamlining of genetic code into life holds …

Cross-genus rebooting of custom-made, synthetic bacteriophage genomes in L-form bacteria

S Kilcher, P Studer, C Muessner… - Proceedings of the …, 2018 - National Acad Sciences
Engineered bacteriophages provide powerful tools for biotechnology, diagnostics, pathogen
control, and therapy. However, current techniques for phage editing are experimentally …

Phage-based applications in synthetic biology

S Lemire, KM Yehl, TK Lu - Annual review of virology, 2018 - annualreviews.org
Bacteriophage research has been instrumental to advancing many fields of biology, such as
genetics, molecular biology, and synthetic biology. Many phage-derived technologies have …

Escherichia coli as a host for metabolic engineering

S Pontrelli, TY Chiu, EI Lan, FYH Chen, P Chang… - Metabolic …, 2018 - Elsevier
Over the past century, Escherichia coli has become one of the best studied organisms on
earth. Features such as genetic tractability, favorable growth conditions, well characterized …

The next generation of synthetic biology chassis: moving synthetic biology from the laboratory to the field

BL Adams - ACS Synthetic Biology, 2016 - ACS Publications
Escherichia coli (E. coli) has played a pivotal role in the development of genetics and
molecular biology as scientific fields. It is therefore not surprising that synthetic biology (SB) …

Chasing bacterial chassis for metabolic engineering: a perspective review from classical to non‐traditional microorganisms

P Calero, PI Nikel - Microbial Biotechnology, 2019 - Wiley Online Library
The last few years have witnessed an unprecedented increase in the number of novel
bacterial species that hold potential to be used for metabolic engineering. Historically …

Enhancing phage therapy through synthetic biology and genome engineering

BR Lenneman, J Fernbach, MJ Loessner, TK Lu… - Current Opinion in …, 2021 - Elsevier
Highlights•Synthetic biology enables efficient phage genome engineering.•Phages with
tunable host range and antimicrobial payload delivery enhance efficacy.•Phage design …