A humanized IgG antibody to CAMPATH‐1 antigen (CDw52) is known to be lympholytic both
in vitro and in vivo. So as to improve therapeutic potency through protein engineering …

Antibody-mediated lysis of cells involves a complex interaction between the cell, the target
antigen, the antibody and host effector mechanisms. One such mechanism, complement …

Of the five immunoglobulin isotypes, immunoglobulin G (IgG) is most abundant in human
serum. The four subclasses, IgG1, IgG2, IgG3, and IgG4, which are highly conserved, differ …

Recombinant human IgG antibodies (hIgGs) completely devoid of binding to Fcγ receptors
(FcγRs) and complement protein C1q, and thus with abolished immune effector functions …

We report here the engineering of a humanized anti-human EphA2 mAb (mAb 12G3H11) in
an effort to explore the relationship between the hinge of a human IgG1 and its effector …

This manuscript describes two sites in a human IgG1 that, when mutated individually or in
combination, result in a dramatic increase in C1q binding and complement-dependent …

Abstract Glycosylation of the IgG-Fc is essential for optimal binding and activation of Fcγ
receptors and the C1q component of complement. However, it has been reported that the …

In this paper we describe the construction of mouse-human IgG3 mutant antibodies
resembling IgG1 in their disulfide bond pattern between the heavy and light chain (H L) …

In this work, we present studies of the covalent structure of human IgG2 molecules. Detailed
analysis showed that recombinant human IgG2 monoclonal antibody could be partially …

Human IgG is produced with C-terminal lysines that are cleaved off in circulation. The
function of this modification was unknown and generally thought not to affect antibody …