There is a need in clinical microbiology and public health for assays that enable sensitive
and rapid detection of infectious agents. Next Generation Sequencing (NGS) is increasingly …

Despite the advanced PCR-based assays available, a fraction of the pediatric respiratory
infections remain unexplained every epidemic season, and there is a perception that novel …

Emerging viral infectious diseases have been a constant threat to global public health in
recent times. In managing these diseases, molecular diagnostics has played a critical role …

Viruses are evolving at an alarming rate, spreading and inconspicuously adapting to cutting-
edge therapies. Therefore, the search for rapid, informative and reliable diagnostic methods …

The advent of next-generation sequencing (NGS) technologies has expanded our ability to
detect and analyze microbial genomes and has yielded novel molecular approaches for …

High-throughput sequencing (HTS) enables most pathogens in a clinical sample to be
detected from a single analysis, thereby providing novel opportunities for diagnosis …

Background Next generation sequencing (NGS) is a new technology that can be used for
broad detection of infectious pathogens and is rapidly becoming an essential platform in …

Multiplex PCR assays for respiratory viruses are widely used in routine diagnostics, as they
are highly sensitive, rapid, and cost effective. However, depending on the assay system …

A large number of viruses can individually and concurrently cause various respiratory
illnesses. Metagenomic sequencing using next-generation sequencing (NGS) technology is …

Background Molecular assays are the gold standard methods used to diagnose viral
respiratory pathogens. Pitfalls associated with this technique include limits to the number of …