Synthesis of Galacto-oligosaccharides in Milk by Using Bifidobacterium bifidum β-galactosidases (Saphera 2600L and Nola Fit 5500) Immobilized on Chitosan Beads

M Bodnár, E Fazekas, T Nagy, N Miltner… - Food and Bioprocess …, 2023 - Springer
The lactose intolerance—as a limiting factor for dairy milk consumption—has a high
prevalence worldwide. Dairy milk and milk-derived products are major sources of multiple …

[HTML][HTML] Immobilization of whole cells of Lactococcus lactis containing high levels of a hyperthermostable β-galactosidase enzyme in chitosan beads for efficient …

L Yu, DJ O'Sullivan - Journal of dairy science, 2018 - Elsevier
Galacto-oligosaccharides (GOS) are prebiotic food ingredients that are proposed to
stimulate the growth of beneficial gut microorganisms, particularly bifidobacteria. Previously …

Batch synthesis of galactooligosaccharides from co-products of milk processing using immobilized β-galactosidase from Bacillus circulans

CR Hackenhaar, LS Spolidoro, EEE Flores… - Biocatalysis and …, 2021 - Elsevier
Milk whey (MW) and milk whey permeate (MWP) constitute the main part of co-products
derived from milk processing. The use of the transgalactosylation activity of β-galactosidases …

[HTML][HTML] Synthesis of prebiotic galactooligosaccharides from lactose using bifidobacterial β-galactosidase (BbgIV) immobilised on DEAE-Cellulose, Q-Sepharose and …

A Osman, S Symeou, V Trisse, KA Watson… - Biochemical …, 2014 - Elsevier
The bifidobacterial β-galactosidase BbgIV was immobilised on DEAE-Cellulose and Q-
Sepharose via ionic binding and on amino-ethyl-and glyoxal-agarose via covalent …

Whey valorization using transgalactosylation activity of immobilized β‐galactosidase

M Simović, A Milivojević, M Ćorović… - … Journal of Food …, 2019 - Wiley Online Library
Whey represents a significant dairy industry by‐product that has recently received due
attention based on the rich nutrient composition and significant transformation potential …

Integrated utilization of dairy whey in probiotic β-galactosidase production and enzymatic synthesis of galacto-oligosaccharides

F Duan, R Zhao, J Yang, M Xiao, L Lu - Catalysts, 2021 - mdpi.com
This work established an integrated utilization of dairy whey in β-galactosidase production
from Lactobacillus bulgaricus and prebiotics synthesis by the probiotic enzyme. A cost …

Development of a process for the production and purification of α-and β-galactooligosaccharides from Bifidobacterium bifidum NCIMB 41171

A Goulas, G Tzortzis, GR Gibson - International Dairy Journal, 2007 - Elsevier
Synthesis of prebiotic α-and β-galactooligosaccharides (GOS) using the whole cells of
Bifidobacterium bifidum NCIMB 41171 was investigated. Determination of α-and β …

High Yield Production of a Soluble Bifidobacterial β-Galactosidase (BbgIV) in E. coli DH5α with Improved Catalytic Efficiency for the Synthesis of Prebiotic …

A Osman, G Tzortzis, RA Rastall… - Journal of agricultural …, 2013 - ACS Publications
The bifidobacterial β-galactosidase (BbgIV) was produced in E. coli DH5α at 37 and 30° C in
a 5 L bioreactor under varied conditions of dissolved oxygen (dO2) and pH. The yield of …

Overexpression and characterization of a novel transgalactosylic and hydrolytic β-galactosidase from a human isolate Bifidobacterium breve B24

SH Yi, I Alli, KH Park, BH Lee - New Biotechnology, 2011 - Elsevier
After the complete gene of a β-galactosidase from human isolate Bifidobacterium breve B24
was isolated by PCR and overexpressed in E. coli, the recombinant β-galactosidase was …

Production of galacto-oligosaccharides from lactose by immobilized β-galactosidase and posterior chromatographic separation

JIS Valero - 2009 - search.proquest.com
Galacto-oligosaccharides (GOS) are non-digestible sugars containing two to five molecules
of galactose and one molecule of glucose connected through glycosidic bonds. They are …