Large synthetic genetic circuits require the simultaneous expression of many regulators.
Deactivated Cas9 (dCas9) can serve as a repressor by having a small guide RNA (sgRNA) …

Genetic circuits require many regulatory parts in order to implement signal processing or
execute algorithms in cells. A potentially scalable approach is to use dC as9, which employs …

Over the past few years, tools that make use of the Cas9 nuclease have led to many
breakthroughs, including in the control of gene expression. The catalytically dead variant of …

The RNA-guided CRISPR-associated Cas9 proteins have been widely applied in
programmable genome recombination, base editing or gene regulation in both prokaryotes …

Engineering cellular phenotypes often requires the regulation of many genes. When using
CRISPR interference, coexpressing many single-guide RNAs (sgRNAs) triggers genetic …

Methods for implementing dynamically‐controlled multi‐gene programs could expand
capabilities to engineer metabolism for efficiently producing high‐value compounds. This …

Programmable gene activation enables fine‐tuned regulation of endogenous and synthetic
gene circuits to control cellular behavior. While CRISPR‐Cas‐mediated gene activation has …

CRISPRi-mediated gene regulation allows simultaneous control of many genes. However,
highly specific sgRNA-promoter binding is, alone, insufficient to achieve independent …

High-throughput CRISPR-Cas9 screens have recently emerged as powerful tools to
decipher gene functions and genetic interactions. Here we use a genome-wide library of …

Programmable control over an addressable global regulator would enable simultaneous
repression of multiple genes and would have tremendous impact on the field of synthetic …