Quantitative Detection of Methanotrophs in Soil by Novel pmoA-Targeted Real-Time PCR Assays

S Kolb, C Knief, S Stubner… - Applied and Environmental …, 2003 - Am Soc Microbiol
Methane oxidation in soils is mostly accomplished by methanotrophic bacteria. Little is
known about the abundance of methanotrophs in soils, since quantification by cultivation …

Identification of active methanotrophs in a landfill cover soil through detection of expression of 16S rRNA and functional genes

Y Chen, MG Dumont, A Cébron… - Environmental …, 2007 - Wiley Online Library
Active methanotrophs in a landfill soil were revealed by detecting the 16S rRNA of
methanotrophs and the mRNA transcripts of key genes involved in methane oxidation. New …

Diversity and activity of methanotrophic bacteria in different upland soils

C Knief, A Lipski, PF Dunfield - Applied and Environmental …, 2003 - Am Soc Microbiol
Samples from diverse upland soils that oxidize atmospheric methane were characterized
with regard to methane oxidation activity and the community composition of methanotrophic …

Detection of methanotrophic bacteria in environmental samples with the PCR

IR McDonald, EM Kenna, JC Murrell - Applied and Environmental …, 1995 - Am Soc Microbiol
We designed PCR primers by using the DNA sequences of the soluble methane
monooxygenase gene clusters of Methylosinus trichosporium OB3b and Methylococcus …

Comparison of pmoA PCR Primer Sets as Tools for Investigating Methanotroph Diversity in Three Danish Soils

DG Bourne, IR McDonald… - Applied and environmental …, 2001 - Am Soc Microbiol
Three particulate methane monooxygenase PCR primer sets (A189-A682, A189-A650, and
A189-mb661) were investigated for their ability to assess methanotroph diversity in soils …

Community‐level analysis: key genes of aerobic methane oxidation

MG Dumont, JC Murrell - Methods in enzymology, 2005 - Elsevier
Aerobic methane‐oxidizing bacteria (methanotrophs) are a diverse group of bacteria that
are currently represented by 13 recognized genera. They play a major role in the global …

In situ measurement of methane fluxes and analysis of transcribed particulate methane monooxygenase in desert soils

R Angel, R Conrad - Environmental Microbiology, 2009 - Wiley Online Library
Aerated soils are a biological sink for atmospheric methane. However, the activity of desert
soils and the presence of methanotrophs in these soils have hardly been studied. We …

Detection, isolation, and characterization of acidophilic methanotrophs from Sphagnum mosses

N Kip, W Ouyang, J van Winden… - Applied and …, 2011 - Am Soc Microbiol
Sphagnum peatlands are important ecosystems in the methane cycle. Methane-oxidizing
bacteria in these ecosystems serve as a methane filter and limit methane emissions. Yet little …

Alpha-and gammaproteobacterial methanotrophs codominate the active methane-oxidizing communities in an acidic boreal peat bog

KC Esson, X Lin, D Kumaresan… - Applied and …, 2016 - Am Soc Microbiol
The objective of this study was to characterize metabolically active, aerobic methanotrophs
in an ombrotrophic peatland in the Marcell Experimental Forest, in Minnesota …

Characterization of methanotrophic bacterial populations in soils showing atmospheric methane uptake

AJ Holmes, P Roslev, IR McDonald… - Applied and …, 1999 - Am Soc Microbiol
The global methane cycle includes both terrestrial and atmospheric processes and may
contribute to feedback regulation of the climate. Most oxic soils are a net sink for methane …