[HTML][HTML] Orientation-controlled electrocatalytic efficiency of an adsorbed oxygen-tolerant hydrogenase

N Heidary, T Utesch, M Zerball, M Horch, D Millo… - PLoS …, 2015 - journals.plos.org
Protein immobilization on electrodes is a key concept in exploiting enzymatic processes for
bioelectronic devices. For optimum performance, an in-depth understanding of the enzyme …

Electrostatic-driven activity, loading, dynamics, and stability of a redox enzyme on functionalized-gold electrodes for bioelectrocatalysis

VP Hitaishi, I Mazurenko, M Harb, R Clément… - Acs …, 2018 - ACS Publications
The oxygen reduction reaction is the limiting step in fuel cells, and many works are in
progress to find efficient cathode catalysts. Among them, bilirubin oxidases are copper …

Elucidating film loss and the role of hydrogen bonding of adsorbed redox enzymes by electrochemical quartz crystal microbalance analysis

VM Badiani, SJ Cobb, A Wagner, AR Oliveira… - Acs …, 2022 - ACS Publications
The immobilization of redox enzymes on electrodes enables the efficient and selective
electrocatalysis of useful reactions such as the reversible interconversion of dihydrogen (H2) …

Oriented immobilization of a membrane-bound hydrogenase onto an electrode for direct electron transfer

C Gutiérrez-Sánchez, D Olea, M Marques… - Langmuir, 2011 - ACS Publications
The interaction of redox enzymes with electrodes is of great interest for studying the catalytic
mechanisms of redox enzymes and for bioelectronic applications. Efficient electron transport …

Toward single-enzyme molecule electrochemistry:[NiFe]-hydrogenase protein film voltammetry at nanoelectrodes

FJM Hoeben, FS Meijer, C Dekker, SPJ Albracht… - ACS …, 2008 - ACS Publications
We have scaled down electrochemical assays of redox-active enzymes enabling us to study
small numbers of molecules. Our approach is based on lithographically fabricated Au …

Modeling of the electrostatic interaction and catalytic activity of [NiFe] hydrogenases on a planar electrode

MA Ruiz-Rodríguez, CD Cooper… - The Journal of …, 2022 - ACS Publications
Hydrogenases are a group of enzymes that have caught the interest of researchers in
renewable energies, due to their ability to catalyze the redox reaction of hydrogen. The …

[HTML][HTML] Controlling redox enzyme orientation at planar electrodes

VP Hitaishi, R Clement, N Bourassin, M Baaden… - Catalysts, 2018 - mdpi.com
Redox enzymes, which catalyze reactions involving electron transfers in living organisms,
are very promising components of biotechnological devices, and can be envisioned for …

Guiding principles of hydrogenase catalysis instigated and clarified by protein film electrochemistry

FA Armstrong, RM Evans, SV Hexter… - Accounts of chemical …, 2016 - ACS Publications
Conspectus Protein film electrochemistry (PFE) is providing cutting-edge insight into the
chemical principles underpinning biological hydrogen. Attached to an electrode, many …

[HTML][HTML] Electrochemistry of surface-confined enzymes: Inspiration, insight and opportunity for sustainable biotechnology

LP Jenner, JN Butt - Current Opinion in Electrochemistry, 2018 - Elsevier
Highlights•New strategies for enzyme electrochemistry.•Recent applications of enzyme
electrochemistry.•Synergies of fundamental and applied enzyme electrochemistry.Redox …

Effect of the protonation degree of a self-assembled monolayer on the immobilization dynamics of a [NiFe] hydrogenase

T Utesch, D Millo, MA Castro, P Hildebrandt, I Zebger… - Langmuir, 2013 - ACS Publications
Understanding the interaction and immobilization of [NiFe] hydrogenases on functionalized
surfaces is important in the field of biotechnology and, in particular, for the development of …